Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocyte maturation☆

Naohiro Hashimoto; Takeo Kishimoto

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Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocyte maturation☆

机译：小鼠卵母细胞成熟过程中细胞质成熟促进因子对减数分裂中期的调控☆

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Duringmouseoocytematurationtheregulationoftheactivityofacytoplasmicmaturation-promotingfactor(MPF)wasexamined.ThemouseMPFactivitywasdeterminedbasedonitsabilitytoinducematurationinimmaturestarfishoocytesaftermicroinjectionwiththecytoplasmfrommouseoocytes.MPFappearedinitiallyatgerminalvesiclebreakdown(GVBD),anditsactivityfluctuatedinexactcorrespondencewithmeioticcycles,reachingapeakateachmetaphaseandalmostdisappearingatthetimeofemissionofthefirstpolarbody.CycloheximideaffectedneithertheinitialMPFappearancenorGVBD.Thereafter,however,inthepresenceofcycloheximidethemeioticspindlewasnotformedandMPFdisappeared,althoughthechromosomesremainedcondensed.Afterremovingcycloheximide,MPFreappearedandwasfollowedbythefirstmetaphaseandsubsequentlybypolarbodyemission.Finallythemeioticcycleprogressedtothesecondmetaphase.Thus,fortheappearanceofMPF,thereisacriticalperiodshortlybeforethefirstmetaphase,afterwhichproteinsynthesisisrequired.InthepresenceofeithercytochalasinDorcolcemid,MPFactivityremainedatelevatedlevels.Additionofcycloheximidetosuchcytochalasin-treatedoocytes,inwhichthemeioticcyclewasarrestedatthefirstmetaphase,causedtheMPFlevelstodecreaseandwasfollowedbymovementofchromosomestobothpoleswheretheydecondensedandtwonucleus-likestructureswereformed.Thus,thedisappearanceofMPFmayinitiatethemetaphase-anaphasetransition.Furthermore,detailedcytologicalexaminationrevealedthatchromosomesincytochalasin-treatedoocytesweremonovalentwhilethosetreatedonlywithcycloheximideweredivalent,suggestingthatdissociationofthesynapsisisaprerequisiteforchromosomedecondensationafterthedisappearanceofMPF.Inalltheserespects,MPFseemstobeametaphase-promotingfactorratherthanjustamaturation-promotingfactor.

机译：Duringmouseoocytematurationtheregulationoftheactivityofacytoplasmicmaturation-promotingfactor（MPF）wasexamined.ThemouseMPFactivitywasdeterminedbasedonitsabilitytoinducematurationinimmaturestarfishoocytesaftermicroinjectionwiththecytoplasmfrommouseoocytes.MPFappearedinitiallyatgerminalvesiclebreakdown（GVBD），anditsactivityfluctuatedinexactcorrespondencewithmeioticcycles，reachingapeakateachmetaphaseandalmostdisappearingatthetimeofemissionofthefirstpolarbody.CycloheximideaffectedneithertheinitialMPFappearancenorGVBD.Thereafter，但是，inthepresenceofcycloheximidethemeioticspindlewasnotformedandMPFdisappeared，althoughthechromosomesremainedcondensed.Afterremovingcycloheximide，MPFreappearedandwasfollowedbythefirstmetaphaseandsubsequentlybypolarbodyemission.Finallythemeioticcycleprogressedtothesecondmetaphase.Thus，fortheappearanceofMPF，thereisacriticalperiodshortlybeforethefirstmetaphase，afterwhichproteinsynthesisisrequired.InthepresenceofeithercytochalasinDorcolcemid，MPFactivityremainedateleva tedlevels.Additionofcycloheximidetosuchcytochalasin-treatedoocytes，inwhichthemeioticcyclewasarrestedatthefirstmetaphase，causedtheMPFlevelstodecreaseandwasfollowedbymovementofchromosomestobothpoleswheretheydecondensedandtwonucleus-likestructureswereformed.Thus，thedisappearanceofMPFmayinitiatethemetaphase-anaphasetransition.Furthermore，detailedcytologicalexaminationrevealedthatchromosomesincytochalasin-treatedoocytesweremonovalentwhilethosetreatedonlywithcycloheximideweredivalent，suggestingthatdissociationofthesynapsisisaprerequisiteforchromosomedecondensationafterthedisappearanceofMPF.Inalltheserespects，MPFseemstobeametaphase-promotingfactorratherthanjustamaturation-promotingfactor。

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《Developmental biology》 |1988年第2期|共页
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Naohiro Hashimoto; Takeo Kishimoto;
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Regulation of meiotic metaphase by a cytoplasmic maturation-promoting factor during mouse oocyte maturation☆

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