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首页> 外文期刊>Journal of Clinical Microbiology >Radioimmunoassay system using a serovar-specific lipopolysaccharide antigen of Leptospira.
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Radioimmunoassay system using a serovar-specific lipopolysaccharide antigen of Leptospira.

机译：使用钩端螺旋体的血清特异性脂多糖抗原的放射免疫分析系统。

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The serovar-specific main (TM) antigen of Leptospira interrogans serovar kremastos strain Kyoto was labeled with sodium boro[3H]hydride after 1 h of oxidation with periodate. When 50 ng of the labeled compound was employed, 50% of the compound was bound to the antibodies contained in 7.5 x 10(-2) nl of anti-kremastos Kyoto serum. Under this condition, only 9 ng of the homologous TM antigen was required for 50% inhibition of serovar kremastos Kyoto 3H-TM antigen-anti-kremastos Kyoto serum binding, whereas 5,000 times as much TM antigen of serovar hebdomadis, which belongs to the same Hebdomadis serogroup as serovar kremastos, was required for the same inhibition. The TM antigens from serovars pomona, icterohaemorrhagiae, and copenhageni, which belong to different serogroups, showed no inhibition in an amount of up to 3 x 10(3), 2 x 10(5), and 2 x 10(5) ng, respectively. In the inhibition study using the serovar hebdomadis TM antigen, inhibition values slightly fluctuated due to the antigen's insolubility. When the TM antigens were solubilized with 0.1% sodium taurodeoxycholate, the fluctuation in inhibition values was minimized, and the cross-reactivity of the serovar hebdomadis TM antigen with anti-kremastos Kyoto serum was diminished, while the inhibitory activity of the serovar kremastos Kyoto TM antigen was enhanced.
机译：用高碘酸盐氧化1小时后，用硼化[3H]氢化钠标记问号钩端螺旋体血清型kremastos京都菌株的血清型特异性主（TM）抗原。当使用50 ng的标记化合物时，50％的化合物与7.5 x 10（-2）nl抗kremastos Kyoto血清中包含的抗体结合。在这种条件下，只需要9 ng的同源TM抗原即可抑制50％血清型kremastos Kyoto 3H-TM抗原与抗kremastos Kyoto血清结合，而血清型横纹肌的TM抗原则是后者的5,000倍相同的抑制作用还需要Hebdomadis血清群作为serovar kremastos。来自血清杆菌，沙门氏菌和哥本哈根的TM抗原，属于不同的血清群，在3 x 10（3），2 x 10（5）和2 x 10（5）ng的量下未显示抑制作用，分别。在使用serovar hebdomadis TM抗原的抑制研究中，由于抗原的不溶性，抑制值略有波动。当TM抗原用0.1％牛磺脱氧胆酸钠溶解时，抑制值的波动最小，并且血清型hebdomadis TM抗原与抗kremastos Kyoto血清的交叉反应性降低，而对kromastos Kyoto TM的抑制活性抗原增强。

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