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首页> 外文期刊>Journal of Clinical Microbiology >Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves.
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Antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves.

机译：某些牛轮状病毒之间的抗原关系：gnotobiotic小牛的血清中和和交叉保护。

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A method was further developed to screen non-tissue-culture-adapted bovine rotaviruses for serotype, using a neutralization test with infectious fecal rotavirus. One of those rotaviruses (B223) which was not blocked by antiserum to the neonatal calf diarrhea virus (NCDV) serotype was then adapted to cell culture in the presence of the antiserum for two or more passages and hyperimmune antiserum to this isolate had a 60-fold-higher homologous neutralization titer than with the NCDV serotype rotavirus. Seventy-three isolates were serotyped and eight (11%) were not of the NCDV serotype (bovine rotavirus serotype I). Of these eight, five belonged to the new bovine rotavirus serotype II and three were not typed, indicating the existence of one or more further serotypes. Cross-protection studies in gnotobiotic calves showed that cross-protection only occurred between rotaviruses of the same serotype, and even a minor serotype difference was sufficient for the calves to show a lack of cross-protection. The serotypes (I and II and the three untyped isolates) also showed differences in the rate of migration in polyacrylamide gel electrophoresis of some of their RNA segments (no. 4, 6, 7, 8, 9, 10), indicating that they were of different electropherotypes.
机译：使用感染性粪便轮状病毒的中和试验，进一步开发了一种筛选非组织培养适应性牛轮状病毒血清型的方法。然后，将一种不受新生牛腹泻病毒（NCDV）血清型抗血清阻断的轮状病毒（B223）中的一种，在存在抗血清的情况下进行两次或更多次传代的细胞培养，对该分离株的超免疫抗血清具有60-同源中和滴度比NCDV血清型轮状病毒高三倍。对73株分离株进行了血清分型，其中8株（11％）不是NCDV血清型（牛轮状病毒I型）。在这八种中，有五种属于新的牛轮状病毒血清型II，而三种尚未分型，表明存在一种或多种其他血清型。 gnotobiotic小牛的交叉保护研究表明，交叉保护仅在相同血清型的轮状病毒之间发生，即使很小的血清型差异也足以使小牛表现出缺乏交叉保护。血清型（I和II和三个未分型的分离株）也显示出其某些RNA片段（第4、6、7、8、9、10号）在聚丙烯酰胺凝胶电泳中迁移速率的差异，表明它们是不同的电信号型。

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