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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Neisseria gonorrhoeae by genetic transformation: a clinical laboratory evaluation.
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Identification of Neisseria gonorrhoeae by genetic transformation: a clinical laboratory evaluation.

机译:通过遗传转化鉴定淋病奈瑟菌:临床实验室评估。

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Transformation of a Neisseria gonorrhoeae auxotroph (uracil and arginine deficient) to prototrophy was attempted with wild-type deoxyribonucleic acid from 71 random clinical N. gonorrhoeae cultures. Of these 71 cultures, 97.1% transformed the nutritionally deficient mutant to prototrophy. The procedure was reliable and economical and offered several distinct advantages over other methods used for the confirmation of N. gonorrhoeae.
机译:尝试用来自71种随机临床淋病奈瑟氏球菌培养物的野生型脱氧核糖核酸将淋病奈瑟氏球菌营养缺陷型(尿嘧啶和精氨酸缺陷)转化为原养型。在这71种培养物中,有97.1%将营养不足的突变体转化为原养型。该方法是可靠且经济的,与用于确认淋病奈瑟氏球菌的其他方法相比,具有一些明显的优势。

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