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首页> 外文期刊>Journal of Clinical Microbiology >Monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of Bordetella pertussis filamentous hemagglutinin.
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Monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of Bordetella pertussis filamentous hemagglutinin.

机译：基于单克隆抗体的三明治酶联免疫吸附法检测百日咳博德特氏菌丝状血凝素。

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摘要

Hybrid cell lines producing monoclonal antibodies against Bordetella pertussis filamentous hemagglutinin (FHA) were established. The specificity of the antibodies was ascertained by enzyme-linked immunosorbent assay (ELISA), sandwich ELISA, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroblotting. The monoclonal antibody-based sandwich ELISA was developed for detection of B. pertussis FHA. The assay had a detection limit of B. pertussis FHA in concentrations ranging from 7 to 15 ng/ml. The assay was also able to detect whole B. pertussis, Bordetella parapertussis, and Bordetella bronchiseptica bacteria. No cross-reactions were observed with strains of Branhamella catarrhalis, Neisseria meningitidis, Haemophilus influenzae, Klebsiella pneumoniae, Legionella pneumophila, Streptococcus miteor, or Streptococcus pneumoniae. The monoclonal antibodies might be useful for the detection of soluble antigens and whole bacteria in clinical samples and for studies of the immunochemical structure of B. pertussis FHA.
机译：建立了产生针对百日咳博德特氏菌丝状血凝素（FHA）的单克隆抗体的杂交细胞系。抗体的特异性通过酶联免疫吸附测定（ELISA），夹心ELISA和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和电印迹法确定。开发了基于单克隆抗体的夹心ELISA，用于检测百日咳博德特氏菌FHA。该测定法对百日咳博德特氏菌FHA的检出限为7至15 ng / ml。该测定法还能够检测整个百日咳博德特氏菌，副百日咳博德特氏菌和支气管博德特氏菌。没有观察到与卡他氏杆菌，脑膜炎奈瑟菌，流感嗜血杆菌，肺炎克雷伯菌，嗜肺军团菌，米特链球菌或肺炎链球菌菌株的交叉反应。单克隆抗体可用于检测临床样品中的可溶性抗原和完整细菌，以及用于研究百日咳博德特氏菌FHA的免疫化学结构。

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