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首页> 外文期刊>Journal of Clinical Microbiology >Determination of brucella immunoglobulin G agglutinating antibody titer with dithiothreitol.
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Determination of brucella immunoglobulin G agglutinating antibody titer with dithiothreitol.

机译：用二硫苏糖醇测定布鲁氏菌免疫球蛋白G凝集抗体滴度。

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The routine brucella agglutination test measures both immunoglobulin M (IgM) and IgG brucella antibody titers; however, only an elevated IgG titer is significant for differentiating active from inactive disease in patients with symptoms lasting 3 or more weeks. The IgG antibody titer can be determined by treating the serum wih 2-mercaptoethanol to inactivate the IgM brucella antibodies while leaving the IgG brucella antibodies intact. Dithiothreitol, which also inactivates IgM, was compared with 2-mercaptoethanol for the determination of IgG brucella agglutination titers. The dithiothreitol and 2-mercaptoethanol test results agreed within +/- 1 dilution step in 103 of 105 serum specimens tested, for a 98.1% rate of agreement. The results indicate that dithiothreitol can be used in place of 2-mercaptoethanol for determining IgG brucella agglutination titers. Dithiothreitol does not have the offensive odor or the irritant properties of 2-mercaptoethanol.
机译：常规布鲁氏菌凝集试验可测量免疫球蛋白M（IgM）和IgG布鲁氏菌抗体滴度。但是，只有持续升高的IgG滴度才有意义，可将症状持续3周或更长时间的患者区分为主动疾病和非活动疾病。可以通过用2-巯基乙醇处理血清以使IgM布鲁氏菌抗体失活，同时保持IgG布鲁氏菌抗体完整来确定IgG抗体的滴度。将还使IgM失活的二硫苏糖醇与2-巯基乙醇进行比较，以确定IgG布氏菌凝集滴度。二硫苏糖醇和2-巯基乙醇的测试结果在105个血清样本中的103个样本的+/- 1稀释步骤中一致，一致率为98.1％。结果表明，二硫苏糖醇可以代替2-巯基乙醇用于测定IgG布鲁氏菌凝集滴度。二硫苏糖醇不具有2-巯基乙醇的刺激性气味或刺激性。

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