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首页> 外文期刊>Journal of Clinical Microbiology >Detection of antigens and antibodies in the urine of humans with Plasmodium falciparum malaria.
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Detection of antigens and antibodies in the urine of humans with Plasmodium falciparum malaria.

机译：恶性疟原虫疟疾在人类尿液中的抗原和抗体检测。

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Humans infected with Plasmodium falciparum frequently have elevated levels of proteins in their urine, but it is unclear if any of these proteins are parasite antigens or antimalarial antibodies. To resolve this question, urine samples from malaria patients and controls living in Thailand and Ghana were evaluated. Urine samples from 85% of the patients had elevated protein levels and contained proteins with Mrs ranging from less than 29,000 to greater than 224,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Antisera were produced against urine from infected and control subjects. Antisera raised against infected, but not control, urine were positive by indirect immunofluorescence on P. falciparum parasites and immunoprecipitated approximately 12 unique bands from extracts of parasites metabolically labeled with 35S-methionine. These data suggest that a variety of P. falciparum antigens are released into urine during acute infection. It is also likely that anti-P. falciparum antibodies are present in the urine of malaria patients because samples from these patients, but not controls, were positive in indirect immunofluorescence assays and immunoprecipitated at least 19 P. falciparum antigens from extracts of metabolically labeled parasites. The detection of malarial antigens and antibodies in urine may lead to a new approach for the diagnosis of malaria.
机译：恶性疟原虫感染的人尿液中的蛋白质水平经常升高，但是尚不清楚这些蛋白质中有否是寄生虫抗原或抗疟抗体。为了解决这个问题，对生活在泰国和加纳的疟疾患者和对照组的尿液样本进行了评估。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定，来自85％的患者的尿液样品中的蛋白质水平升高，并且其Mrs范围从小于29,000到大于224,000。针对感染和对照对象的尿液产生抗血清。通过对恶性疟原虫的间接免疫荧光检测，抗血清对感染的尿液（而非对照尿液）呈阳性反应，并从用35S-蛋氨酸代谢标记的寄生虫提取物中免疫沉淀出约12条独特的条带。这些数据表明在急性感染期间，多种恶性疟原虫抗原释放到尿中。反P也可能。疟疾患者尿液中存在恶性疟原虫抗体，因为这些患者（而非对照）的样品在间接免疫荧光测定中呈阳性，并且从代谢标记的寄生虫提取物中免疫沉淀了至少19种恶性疟原虫抗原。尿液中疟疾抗原和抗体的检测可能会导致一种新的疟疾诊断方法。

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