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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the Penner and Lior methods for serotyping Campylobacter spp.
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Comparison of the Penner and Lior methods for serotyping Campylobacter spp.

机译：Penner和Lior方法对弯曲杆菌属进行血清分型的比较。

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摘要

We compared two Campylobacter serotyping systems by using 1,405 isolates of Campylobacter collected from human, animal, and environmental sources during epidemiologic investigations and special studies. We found 96.1% of isolates to be typable by the Penner method for heat-stable antigens, which involved the use of an indirect hemagglutination technique, and 92.1% of isolates to be typable by the Lior method for heat-labile antigens, which involved the use of a slide agglutination technique and absorbed antisera. Absorbed antisera were not required for the Penner method, making that method less difficult to implement. The Lior method was simpler to perform and gave more rapid results than did the Penner method. Cultures frequently reacted in multiple antisera with the Penner method, whereas multiple reactions were rare with the Lior method. Thus, results were easier to interpret with the Lior system. Strains of a single serotype in one system were sometimes found to be multiple serotypes in the other system; hence, the two methods have the potential to be complementary. Both systems were comparable in serotyping isolates from human and nonhuman sources and for evaluating the relationship of strains collected during outbreak investigations.
机译：在流行病学调查和特殊研究中，我们使用从人，动物和环境来源收集的1,405株弯曲杆菌菌株，比较了两种弯曲杆菌血清分型系统。我们发现96.1％的分离株可通过Penner方法对热稳定抗原进行分型，这涉及使用间接血凝技术，而92.1％的分离株可通过Lior方法对热不稳定抗原进行分型，其中包括使用玻片凝集技术和吸收的抗血清。 Penner方法不需要吸收抗血清，因此该方法难以实施。与Penner方法相比，Lior方法执行起来更简单，并且结果更快。潘纳（Penner）方法经常在多种抗血清中发生培养反应，而李尔（Lior）方法很少发生多种反应。因此，使用Lior系统更容易解释结果。在一个系统中，单一血清型的菌株有时在另一系统中被发现为多种血清型。因此，这两种方法有可能互补。两种系统在人类和非人类来源的血清分型分离物中以及在暴发调查期间评估所收集菌株之间的关系方面均具有可比性。

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