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首页> 外文期刊>Journal of Clinical Microbiology >Characterization of La Crosse Virus RNA in Autopsied Central Nervous System Tissues
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Characterization of La Crosse Virus RNA in Autopsied Central Nervous System Tissues

机译：尸体中枢神经系统组织中的La Crosse病毒RNA的表征

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A reverse transcription-PCR (RT-PCR) technique was used to detect La Crosse (LAC) virus RNA in the central nervous system (CNS) tissues of two patients who died of LAC encephalitis in 1960 and 1978. Viral RNA was readily detected by RT-PCR although the tissues had been stored frozen for up to 37 years. LAC virus was detected in the cerebral cortex but not in other CNS tissues. RT-PCR allowed detection of replicative forms of the virus, indicating that the virus was actively replicating in the specific CNS tissues. The small (S) RNA segments of the viruses from the CNS samples were demonstrated to be genetically similar by single-strand conformation polymorphism analyses. These S RNA segments were then sequenced; only two base changes were demonstrated between the 1960 and the 1978 samples, suggesting that LAC virus is genetically stable in areas of endemicity. The RT-PCR analyses of analyte directly from CNS tissues allows study of the virus without passage in cell culture.
机译：逆转录PCR（RT-PCR）技术用于检测1960年和1978年死于LAC脑炎的两名患者的中枢神经系统（CNS）组织中的La Crosse（LAC）病毒RNA。 RT-PCR尽管组织已冷冻保存长达37年。 LAC病毒在大脑皮层中检测到，但在其他CNS组织中未检测到。 RT-PCR可以检测病毒的复制形式，表明该病毒正在特定的CNS组织中活跃复制。通过单链构象多态性分析，证明来自CNS样品的病毒小（S）RNA片段在遗传上相似。然后对这些S RNA片段进行测序。在1960年至1978年的样本之间仅显示了两个基本变化，这表明LAC病毒在地方性地区具有遗传稳定性。直接从CNS组织中进行分析物的RT-PCR分析，可以研究病毒而无需在细胞培养中传代。

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