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首页> 外文期刊>Journal of Clinical Microbiology >Isolation and characterization of monoclonal antibodies against alkaline phosphatase of Pseudomonas aeruginosa.
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Isolation and characterization of monoclonal antibodies against alkaline phosphatase of Pseudomonas aeruginosa.

机译：铜绿假单胞菌碱性磷酸酶单克隆抗体的分离与鉴定。

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摘要

Monoclonal antibodies against the alkaline phosphatase of Pseudomonas aeruginosa were produced from spleen cells of BALB/c mice primed with purified alkaline phosphatase of P. aeruginosa ATCC 10145 and SP20/Ag-14 myeloma cells. The eight stable clones established produced antibodies that reacted by enzyme-linked immunosorbent and indirect immunofluorescence assays with all bacterial strains of P. aeruginosa, including the 17 serotypes and two nontypable strains. Three of the clones cross-reacted only with some Pseudomonas species of the rRNA homology group I defined by N. J. Palleroni (in N. R. Krieg and J. G. Holt, ed., Bergey's Manual of Systematic Bacteriology, 8th ed., p. 140-218, 1984). The other clones also interacted with other species, including Pseudomonas acidovorans and Xanthomonas maltophilia. Because other species of the genera Aeromonas and Acinetobacter and species of the family Enterobacteriaceae were not detected by these monoclonal antibodies, the antibodies could be used as reagents for routine detection of P. aeruginosa in clinical specimens. Interactions of the antibodies with other Pseudomonas species such as P. fluorescens and P. stutzeri are not important, since these species are susceptible to the same antipseudomonal agents.
机译：从铜绿假单胞菌ATCC 10145和SP20 / Ag-14骨髓瘤细胞的纯化碱性磷酸酶引发的BALB / c小鼠脾细胞中产生了针对铜绿假单胞菌碱性磷酸酶的单克隆抗体。建立的八个稳定克隆产生了抗体，这些抗体通过酶联免疫吸附和间接免疫荧光分析与铜绿假单胞菌的所有细菌菌株（包括17种血清型和2种不可分型的菌株）反应。其中三个克隆仅与NJ Palleroni定义的rRNA同源群I的某些假单胞菌属物种发生交叉反应（在NR Krieg和JG Holt编辑，Bergey的《系统细菌学手册》，第8版，第140-218页，1984年） ）。其他克隆也与其他物种相互作用，包括嗜酸假单胞菌和嗜黄单胞菌。由于这些单克隆抗体未检测到气单胞菌和不动杆菌属的其他种以及肠杆菌科的种，因此该抗体可用作常规检测临床标本中的铜绿假单胞菌的试剂。抗体与荧光假单胞菌和斯氏假单胞菌等其他假单胞菌物种之间的相互作用并不重要，因为这些物种对相同的抗假单胞菌剂敏感。

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