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首页> 外文期刊>Journal of Clinical Microbiology >Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis.
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Enzyme-linked immunosorbent assay for immunoglobulin M specific antibody for the diagnosis of melioidosis.

机译：免疫球蛋白M特异性抗体的酶联免疫吸附测定，用于诊断类li疮。

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Indirect hemagglutination (IHA) is commonly used for serodiagnosis of melioidosis. However, in endemic areas, high background titers in normal populations and occasional low titers in patients with septicemic melioidosis prompted a search for a more sensitive and more specific method of serodiagnosis. An indirect fluorescent-antibody test for immunoglobulin M (IgM) specific antibody to Pseudomonas pseudomallei was more sensitive and more specific, but fluorescence microscopes are rarely available in the endemic areas. An enzyme-linked immunosorbent assay (ELISA) for IgM antibody is an attractive alternative. An indirect ELISA for IgM antibody (IgM ELISA) and an IgM antibody capture ELISA for melioidosis were developed. Both tests, together with IHA, were evaluated for 153 serum specimens from blood donors and 16 serum specimens from 16 melioidosis patients. It was found that IHA, the IgM ELISA, and the IgM antibody capture ELISA had sensitivities of 88, 88, and 75%, respectively, with specificities of 97.4, 92.2, and 91.5%, respectively. When IHA was combined with IgM ELISA, a sensitivity of 100% and a specificity of 95.4% were obtained. The IgM ELISA and IHA should be used in combination for serodiagnosis of melioidosis.
机译：间接血凝（IHA）通常用于类鼻疽的血清诊断。但是，在流行地区，正常人群中本底滴度较高，而败血性类拟oid虫病患者偶尔滴度较低，这促使人们寻求一种更敏感，更具体的血清诊断方法。针对假假单胞菌的免疫球蛋白M（IgM）特异性抗体的间接荧光抗体检测更敏感，更特异性，但在流行地区很少使用荧光显微镜。 IgM抗体的酶联免疫吸附测定（ELISA）是一种有吸引力的选择。开发了用于IgM抗体的间接ELISA（IgM ELISA）和用于类li虫病的IgM抗体捕获ELISA。两项测试与IHA一起，评估了153位来自献血者的血清标本和16位来自类鼻oid病患者的16位血清标本。发现IHA，IgM ELISA和IgM抗体捕获ELISA的灵敏度分别为88、88和75％，特异性分别为97.4、92.2和91.5％。当IHA与IgM ELISA结合使用时，灵敏度为100％，特异性为95.4％。 IgM ELISA和IHA应结合用于类鼻疽的血清诊断。

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