...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Analysis of the humoral response to the flagellin protein of Borrelia burgdorferi: cloning of regions capable of differentiating Lyme disease from syphilis.
【24h】

Analysis of the humoral response to the flagellin protein of Borrelia burgdorferi: cloning of regions capable of differentiating Lyme disease from syphilis.

机译：分析对伯氏疏螺旋体鞭毛蛋白的体液反应：克隆能够区分梅毒的莱姆病的区域。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Selected regions of the Borrelia burgdorferi flagellin gene (fla) that exhibit high or low homology with related genes from other bacterial species were amplified by the polymerase chain reaction and expressed as fusion proteins in Escherichia coli. Purified fusion proteins were assayed for antibody reactivity in a microtiter plate enzyme-linked immunosorbent assay with sera from Lyme disease patients as well as syphilitic and normal sera. Immunoglobulin G antibody from Lyme disease patient sera reacted predominantly with the central portion of the protein. The region of the flagellin protein encompassing amino acids 64 to 311 detected nearly all of the immunoglobulin G-positive Lyme sera and only reacted with 1 of 26 syphilis patient serum samples. In contrast, 12 of 26 syphilis patient serum samples and 2 of 47 normal serum samples reacted with the amino terminus of the flagellin protein, whereas 4 of 26 syphilis patient serum samples and 7 of 47 normal serum samples reacted with the carboxyl terminus. The central region containing amino acids 64 to 311 may be employed diagnostically to differentiate antibodies to B. burgdorferi from antibodies to Treponema pallidum. In addition, this region also was recognized by immunoglobulin M in the Lyme patient sera, indicating its potential usefulness as a marker for early Lyme disease.
机译：通过聚合酶链反应扩增与其他细菌相关基因高或低同源性的伯氏疏螺旋体鞭毛蛋白基因（fla）的选定区域，并在大肠杆菌中表达为融合蛋白。在微量滴定板酶联免疫吸附试验中，用莱姆病患者的血清以及梅毒和正常血清检测纯化的融合蛋白的抗体反应性。来自莱姆病患者血清的免疫球蛋白G抗体主要与蛋白质的中央部分反应。鞭毛蛋白蛋白区域包含64至311位氨基酸，几乎可以检测到所有免疫球蛋白G阳性莱姆血清，并且仅与26例梅毒患者血清样品中的1种发生反应。相比之下，梅毒患者血清样品中的12个和正常血清样品中的12个与鞭毛蛋白的氨基末端反应，而梅毒患者血清26个样品中的4个和47个正常血清样品中的7个与羧基末端反应。包含64-311位氨基酸的中央区域可用于诊断，以区分抗B.burgdorferi的抗体和抗梅毒螺旋体的抗体。此外，该区域在莱姆病患者血清中也被免疫球蛋白M识别，表明其作为早期莱姆病标志物的潜在用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号