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首页> 外文期刊>Journal of Clinical Microbiology >Detection of influenza C virus by using an in situ esterase assay.
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Detection of influenza C virus by using an in situ esterase assay.

机译：通过使用原位酯酶测定法检测丙型流感病毒。

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A variety of chemically defined compounds were tested to characterize the substrate specificity of the influenza C virus esterase and to determine whether a substrate could be found that would be useful in an assay to detect the virus. Two new substrates, alpha-naphthyl acetate and alpha-naphthyl propionate, were identified; alpha-naphthyl acetate was employed to develop an assay specific for influenza type C virus in MDCK cells. The assay was sufficiently sensitive to detect esterase activity in a single cell and distinguished influenza C virus infections from those of types A and B viruses. Infected cells could be detected as early as 8 h postinfection, with maximal enzyme detection occurring at 24 h. Assay of influenza C virus in the chorioallantoic or amniotic fluid of infected eggs was performed by applying fluids directly onto nitrocellulose strips and then incubating with alpha-naphthyl acetate. Both the cellular and nitrocellulose-bound assays are rapid, inexpensive, and easy to perform, offering advantages for use in clinical laboratories.
机译：测试了多种化学成分确定的化合物，以表征C型流感病毒酯酶的底物特异性，并确定是否可以找到可用于检测病毒的底物。确定了两种新的底物，乙酸α-萘酯和丙酸α-萘酯。使用α-萘乙酸乙酸酯开发针对MDCK细胞中C型流感病毒的特异性检测方法。该测定法足够灵敏，可以检测单个细胞中的酯酶活性，并且可以将A型和B型流感病毒与C型流感病毒感染区分开。感染后的细胞最早可在感染后8 h被检测到，最大的酶检测在24 h发生。通过将液体直接涂在硝酸纤维素条上，然后与乙酸α-萘酯一起进行孵育，可检测感染卵的绒毛膜尿囊或羊水中的丙型流感病毒。结合细胞和硝酸纤维素的测定均快速，廉价且易于执行，为临床实验室提供了优势。

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