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首页> 外文期刊>Journal of Clinical Microbiology >Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep
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Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep

机译：健康的澳大利亚屠宰年龄羊的产志贺毒素的大肠杆菌的毒力性质和血清型

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A group of 1,623 ovine fecal samples recovered from 65 geographically distinct mutton sheep and prime lamb properties across New South Wales, Australia, were screened for the presence of Shiga toxin-producing Escherichia coli (STEC) virulence factors (stx 1, stx 2,eaeA, and ehxA). A subset was cultured for STEC isolates containing associated virulence factors (eaeAand/or ehxA), which were isolated from 17 of 20 (85%) and 19 of 20 (95%) tested prime lamb and mutton sheep properties, respectively. STEC isolates containing stx 1,stx 2, and ehxA were most commonly isolated (19 of 40 flocks; 47.5%), and this profile was observed for 10 different serotypes. Among 90 STEC isolates studied, the most common serotypes were O91:H? (22 isolates [24.4%]), O5:H? (16 isolates [17.8%]), O128:H2 (11 isolates [12.2%]), O123:H? (8 isolates [8.9%]), and O85:H49 (5 isolates [5.6%]). Two isolates (2.2%) were typed as O157:H?. A total of 78 of 90 STEC isolates (86.7%) expressed Shiga toxin in Vero cell culture and 75 of 84ehxA-positive isolates (89.3%) expressed enterohemolysin on washed sheep blood agar. eaeA was observed in 11 of 90 (12.2%) ovine STEC isolates, including serotypes O5:H?, O84:H?, O85:H49, O123:H? O136:H40, and O157:H?. Although only 2 of 90 isolates were typed as O157:H?, the predominant serotypes recovered during this study have been recovered from human patients with clinical disease, albeit rarely.
机译：筛选了从澳大利亚新南威尔士州65个地理上不同的羊肉和主要羔羊属性中回收的1,623个绵羊粪便样本，以检查是否存在产生志贺毒素的大肠杆菌（STEC）毒力因子（ stx 1 ， stx 2 ， eaeA 和 ehxA ）。培养一个亚组以分离包含相关毒力因子（ eaeA 和/或 ehxA ）的STEC分离株，这些分离自20个中的17个（85％）和20个中的19个（95 ％）分别测试了初等羊羔和羊肉绵羊的特性。包含 stx 1 ， stx 2 和 ehxA 的STEC分离株最常见（40个鸡群中的19个; 47.5％），并且针对10种不同的血清型观察到了这种分布。在研究的90个STEC菌株中，最常见的血清型为O91：H ？（22个菌株[24.4％]），O5：H ？（16个菌株[17.8％]）。 ，O128：H2（11个分离物[12.2％]），O123：H α（8个分离物[8.9％]）和O85：H49（5个分离物[5.6％]）。两个分离株（2.2％）的类型为O157：H ？。 90个STEC分离株中有78个（86.7％）在Vero细胞培养物中表达了志贺毒素，84个ehxA阳性分离株中的75个（89.3％）在洗涤后的羊血琼脂上表达了肠溶血素。在90个（12.2％）绵羊STEC分离株中有11个（ eaeA ）被观察到，包括血清型O5：H ？，O84：H ？，O85： H49，O123：H ？ O136：H40和O157：H ？。尽管在90个分离株中只有2个被定型为O157：H ？，但在这项研究中回收的主要血清型已从患有临床疾病的人类患者中恢复，尽管很少。

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