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首页> 外文期刊>Journal of Clinical Microbiology >Hemagglutination Typing of Escherichia coli: Definition of Seven Hemagglutination Types
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Hemagglutination Typing of Escherichia coli: Definition of Seven Hemagglutination Types

机译：大肠杆菌的血凝分型：七个血凝类型的定义

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A hemagglutination (HA) typing system has been developed for demonstrating and characterizing the mannose-sensitive and mannose-resistant hemagglutinins produced by Escherichia coli isolated from human sources. HA typing is performed by testing CFA agar-grown E. coli cells for HA with human, bovine, adult chicken, African Green monkey, and guinea pig erythrocytes in the presence and absence of mannose. Seven major HA types, designated HA type I through HA type VII, have been defined according to the HA patterns produced by 1,334 test cultures consisting of 33 colonization factor antigen I (CFA/I)-positive enterotoxigenic E. coli (ETEC), 37 CFA/II-positive ETEC, 614 isolates belonging to the classical enteropathogenic E. coli, or EPEC, serogroups, 446 non-ETEC, non-EPEC stool isolates, and 204 bacteremia-associated E. coli. Facultatively enteropathogenic E. coli (FEEC) serogroups, which are the causative agents of extraintestinal infections but also sporadic cases of enteritis, comprised 38% of the stool isolates and 91% of the blood isolates examined. Previous observations concerning the HA patterns of CFA-positive ETEC and the EPEC were confirmed. A significant correlation was found between FEEC serogroups and the production of mannose-resistant HA with human, monkey, and usually chicken erythrocytes (the HA patterns designated HA type VI). A large majority (80.2%) of the FEEC strains belonging to the most frequently isolated serogroups from cases of bacteremia (O1, O2, O4, O6, O7, and O18) produced type VI HA patterns. Stool isolates belonging to these same serogroups were 59.2% positive for HA type VI patterns. In contrast, only 17.4% of the non-FEEC stool isolates and 1.9% of the EPEC isolates belong to HA type VI. Of the blood isolates, the HA type VI phenotype was two times more prevalent among K1-positive E. coli than among K1-negative E. coli, 70.6 versus 31.1%. These results suggest that surface-associated hemagglutinins of E. coli, many of which are known to be fimbriae, should be considered in addition to serotype (O:K:H antigenicity) in the description of isolates.
机译：已开发了血凝（HA）分型系统，以证明和表征由人类来源分离的大肠杆菌产生的对甘露糖敏感和抗甘露糖的血凝素。通过测试CFA琼脂生长的 E进行HA分型。在存在和不存在甘露糖的情况下，人，牛，成年鸡，非洲绿猴和豚鼠红细胞中的HA细胞根据由1334种由33种定殖因子抗原I（CFA / I）阳性肠毒素E E组成的测试培养物产生的HA模式，已定义了7种主要的HA类型，分别命名为I型至HA型。大肠杆菌（ETEC），37种CFA / II阳性ETEC，614株属于经典肠致病性 E。大肠埃希菌或EPEC，血清群，446种非ETEC，非EPEC粪便分离株和204种与菌血症相关的 E。大肠杆菌。肠致病性大肠杆菌。大肠杆菌（FEEC）血清群既是肠外感染的致病菌，也是偶发性肠炎的病例，占所检查的粪便分离株的38％和血液分离株的91％。先前关于CFA阳性ETEC和EPEC的HA模式的观察结果得到确认。发现FEEC血清群与人，猴和通常是鸡的红细胞产生抗甘露糖的HA之间存在显着相关性（HA模式指定为VI型HA）。大部分（80.2％）的FEEC菌株属于从细菌血症病例（O1，O2，O4，O6，O7和O18）中最常分离的血清群，产生VI HA型。属于这些相同血清群的粪便分离株的HA型VI模式阳性59.2％。相反，只有17.4％的非FEEC粪便分离株和1.9％的EPEC分离株属于HA型VI。在这些血液分离物中，HA型VI表型在K1阳性 E中普遍存在两倍。大肠杆菌比K1阴性 E大肠杆菌为70.6比31.1％。这些结果表明 E的表面相关血凝素。在分离株的描述中，除了血清型（O：K：H抗原性）外，还应考虑许多已知为菌毛的大肠埃希菌。

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