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首页> 外文期刊>Journal of Clinical Microbiology >Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism.
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Experimental reproduction of Potomac horse fever in horses with a newly isolated Ehrlichia organism.

机译：在新分离出的埃希氏菌生物体中的马中波托马克马热的实验繁殖。

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摘要

Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infected horses. The agent was continuously propagated in mouse macrophage cell cultures. The organism multiplied in the cytoplasm of mouse macrophage cells and was identified by Giemsa staining, acridine orange staining, and by indirect immunofluorescence with convalescent sera from infected horses. The disease was experimentally reproduced in horses inoculated with Ehrlichia-infected cell culture material. The Ehrlichia organism was reisolated from the blood of these infected horses during the course of the disease. Antibody against the organism was detected in the sera of experimentally infected horses. This study confirmed that the new Ehrlichia organism is the etiological agent of Potomac horse fever.
机译：研究了波托马克马热，这是一种近来公认的马病，其特征是高烧，白细胞减少症和大量腹泻。从感染马血的单核细胞中，在马巨噬细胞-成纤维细胞培养物和小鼠巨噬细胞培养物中分离出埃希氏菌属生物。该试剂在小鼠巨噬细胞培养物中连续繁殖。该生物体在小鼠巨噬细胞的细胞质中繁殖，并通过Giemsa染色，a啶橙染色以及通过感染马匹的恢复期血清的间接免疫荧光进行鉴定。该疾病是在接种了埃里希氏菌感染的细胞培养材料的马中实验性繁殖的。在疾病过程中，从这些被感染的马的血液中分离出埃希氏菌生物。在经过实验感染的马的血清中检测到了针对该生物的抗体。这项研究证实，新的埃希氏菌是波托马克马热的病原体。

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