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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak.
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Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak.

机译：MIDI Sherlock系统和脉冲场凝胶电泳在表征近期医院爆发的耐甲氧西林金黄色葡萄球菌菌株中的比较。

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An outbreak of methicillin-resistant Staphylococcus aureus infections at the University of Utah Health Sciences Center occurred over a 7-month period. While the isolates phenotypically appeared to be similar in gross morphology and have similar Vitek antibiotic susceptibility patterns, two additional methods of strain characterization were evaluated to enhance the epidemiological investigation: pulsed-field gel electrophoresis and gas chromatography with the MIDI Sherlock system. Sherlock uses gas chromatography to qualitatively and quantitatively analyze the cellular fatty acid composition of organisms and creates two-dimensional plots based on principal-component analysis to define groups of closely related organisms. All isolates were also evaluated by digesting their chromosomal DNAs with the low-frequency-cutting enzyme SmaI and separating the restriction fragments by contour-clamped homogeneous electric field gel electrophoresis. Sample preparation for this pulsed-field gel electrophoresis included a novel cell lysis procedure involving achromopeptidase, greatly reducing the turnaround time. Isolates tested were recovered from the following: 45 suspected outbreak patients, 6 hospitalized patients believed to be unrelated to the outbreak, 6 patients from outside the hospital, and one health care practitioner implicated in the outbreak. Of 45 phenotypically similar suspect strains, 43 clustered tightly on the Sherlock two-dimensional plot. All outbreak patient isolates were also identical by pulsed-field gel electrophoresis with the exception of the same two outliers identified by Sherlock. In this epidemiologic investigation, we found an excellent correlation between the Sherlock and pulsed-field gel electrophoresis results for strain characterization of methicillin-resistant S. aureus.
机译：犹他大学健康科学中心爆发了耐甲氧西林金黄色葡萄球菌感染的暴发，历时7个月。尽管这些分离物在表型上总体上看起来相似并且具有相似的Vitek抗生素敏感性模式，但仍评估了另外两种菌株表征方法以增强流行病学研究：脉冲场凝胶电泳和MIDI Sherlock系统气相色谱。 Sherlock使用气相色谱法定性和定量地分析了生物的细胞脂肪酸组成，并基于主成分分析创建了二维图，以定义密切相关的生物群。还通过用低频切割酶SmaI消化它们的染色体DNA并通过轮廓钳制的均匀电场凝胶电泳分离限制性片段来评估所有分离株。此脉冲场凝胶电泳的样品前处理包括一种涉及染色体肽酶的新型细胞裂解程序，大大缩短了周转时间。从以下对象中回收了测试的分离株：45名疑似暴发患者，6名与暴发无关的住院患者，6名来自医院外的患者以及一名与暴发有牵连的卫生保健从业人员。在45个表型相似的可疑菌株中，有43个紧密聚集在Sherlock二维图中。除通过Sherlock鉴定出的两个异常值外，所有爆发患者分离株也均通过脉冲场凝胶电泳相同。在这项流行病学调查中，我们发现了耐福尔的金黄色葡萄球菌菌株鉴定的Sherlock脉冲场电泳结果与脉冲场凝胶电泳结果之间存在极好的相关性。

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