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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Analysis by Pulsed-Field Gel Electrophoresis and Antibiogram of Streptococcus pneumoniae Serotype 6B Isolates from Selected Areas within the United States
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Molecular Analysis by Pulsed-Field Gel Electrophoresis and Antibiogram of Streptococcus pneumoniae Serotype 6B Isolates from Selected Areas within the United States

机译：通过脉冲场凝胶电泳和抗菌素从美国部分地区的肺炎链球菌血清型6B分离株进行分子分析。

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Fifty-eight clinical isolates of Streptococcus pneumoniae serotype 6B, including 16 from Alaska, 14 from Arizona, 11 from Washington, and 17 from seven additional states, were analyzed. The antibiograms of these isolates were assigned to 10 antibiotic profiles based on their susceptibilities to penicillin, erythromycin, tetracycline, and trimethoprim-sulfamethoxazole. Thirty-two (55%) of these isolates were penicillin nonsusceptible, while 21 (36%) were intermediate or resistant to three or more antibiotics. The restriction endonucleases ApaI and SmaI were used to digest intact chromosomes, and the fragments were resolved by pulsed-field gel electrophoresis (PFGE). The ApaI and SmaI PFGE patterns were combined, and 13 of the 16 Alaskan isolates showed indistinguishable PFGE patterns. One other isolate exhibited highly related ApaI and SmaI PFGE patterns, differing by only one band after restriction with ApaI. Among the 14 isolates from Arizona, 1 was indistinguishable from the predominantApaI and SmaI PFGE patterns seen in the Alaskan isolates; 5 others were highly related (±1 band after cutting with either enzyme) to the Alaskan isolates, suggesting a common ancestral origin. Of the remaining eight isolates, six additional ApaI plus SmaI PFGE patterns were observed. The 28 isolates from the various contiguous states had 22ApaI plus SmaI PFGE patterns. No correlations were found between specific PFGE patterns, antibiograms, dates of isolation, or geography. The serotype 6B isolates across the contiguous United States were genetically diverse, while the 6B isolates from Alaska appeared to be much less diverse.
机译：分析了58种6B型肺炎链球菌临床分离株，包括来自阿拉斯加的16个，来自亚利桑那州的14个，来自华盛顿的11个以及来自其他七个州的17个。根据它们对青霉素，红霉素，四环素和甲氧苄氨嘧啶-磺胺甲基恶唑的敏感性，将这些分离物的抗菌谱分为10种抗生素谱。这些分离株中有32（55％）种对青霉素不敏感，而21种（36％）对三种或更多种抗生素具有中等或耐药性。用限制性核酸内切酶 Apa I和 Sma I消化完整的染色体，并通过脉冲场凝胶电泳（PFGE）分离片段。结合了 Apa I和 Sma I PFGE模式，在16个阿拉斯加分离株中，有13个显示出无法区分的PFGE模式。另一种分离株表现出高度相关的 Apa I和 Sma I PFGE模式，在 Apa I限制性酶切后仅相差一个带。在来自亚利桑那州的14种分离株中，有1种与阿拉斯加分离株中主要的 Apa I和 Sma I PFGE模式没有区别。另外5个与阿拉斯加分离物高度相关（用任一酶切割后均出现±1条带），表明其祖先起源相同。在其余的八个分离株中，还观察到六个另外的 Apa I加 Sma I PFGE模式。来自不同连续状态的28个分离株具有22 Apa I加 Sma I PFGE模式。在特定的PFGE模式，抗菌素图，隔离日期或地理位置之间未发现相关性。整个美国的血清型6B分离株在遗传上是多样的，而来自阿拉斯加的6B分离株似乎没有那么多。

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