...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Changes in antibody avidity after virus infections: detection by an immunosorbent assay in which a mild protein-denaturing agent is employed.
【24h】

Changes in antibody avidity after virus infections: detection by an immunosorbent assay in which a mild protein-denaturing agent is employed.

机译：病毒感染后抗体亲和力的变化：通过免疫吸附试验检测，其中使用了温和的蛋白质变性剂。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In titrating serum immunoglobulin G antibody to viruses by enzyme-linked immunosorbent assay, we used two rows of wells for serial twofold dilutions of the serum; in one row, a low concentration of a protein denaturant, 0.5 or 1.0 M guanidine hydrochloride, was added to the diluent so that the binding of low-avidity antibodies to viral antigens on the solid phase was inhibited. We then compared the antibody titration curves obtained in the two rows. We found that the addition of the reagent resulted in a parallel leftward shift of the curves and that the extent of the shift was greater in early than in late sera from all of the three infections studied (Japanese encephalitis virus, rotavirus, and rubella virus infections). This procedure may be useful for estimation of the avidity of antibody in serum and, with further evaluation, may prove to be applicable to single-serum diagnosis of virus infections.
机译：通过酶联免疫吸附法滴定针对病毒的血清免疫球蛋白G抗体时，我们使用两排孔连续两次稀释血清。在一排中，将低浓度的蛋白质变性剂0.5或1.0 M盐酸胍加入稀释液中，从而抑制了低亲和力抗体与固相上病毒抗原的结合。然后，我们比较了两行中获得的抗体滴定曲线。我们发现，试剂的添加导致曲线平行向左移动，并且在所有研究的三种感染（日本脑炎病毒，轮状病毒和风疹病毒感染）中，血清的移动程度早于后期血清。 ）。该程序可能有助于评估血清中抗体的亲和力，并且经过进一步评估，可以证明适用于病毒感染的单血清诊断。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号