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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of a neutralization enzyme immunoassay and an enzyme-linked immunosorbent assay for evaluation of immune status of children vaccinated for mumps.
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Comparison of a neutralization enzyme immunoassay and an enzyme-linked immunosorbent assay for evaluation of immune status of children vaccinated for mumps.

机译：中和酶免疫测定法和酶联免疫吸附测定法在评估腮腺炎疫苗接种儿童的免疫状况方面的比较。

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A 50% neutralization enzyme immunoassay (N50-EIA) was compared with an indirect enzyme-linked immunosorbent assay (ELISA) for determining mumps virus antibodies in three consecutive serum samples from 138 children vaccinated with a live mumps vaccine at the age (in years) of 1.5. By the N50-EIA, most (132 of 138) preserum samples did not show neutralizing activity. Eight to 12 weeks after vaccination, 17 of the children were still negative, but only 7 remained so after 2.5 years, resulting in a seroconversion rate of 125 of 132 (95%). Over the same period, the neutralization geometric mean titer rose from 3.6 to 9.9. By an indirect ELISA, 128 of 138 preserum samples were found negative. The early and late postvaccination sera of 8 children were ELISA negative, resulting in a seroconversion rate of 120 of 128 (94%). Only two children remained seronegative by both methods. Seven of the late postvaccination serum samples yielded noncorresponding results, reflecting 95% correlation between both methods. Due to cross-reactivity with parainfluenza viruses, the ELISA proved to be less specific, but on the other hand, it showed a greater sensitivity than the N50-EIA.
机译：将50％中和酶免疫测定法（N50-EIA）与间接酶联免疫吸附测定法（ELISA）进行了比较，以测定138岁年龄段（岁）中接种活腮腺炎疫苗的儿童连续三个血清样本中的腮腺炎病毒抗体1.5。通过N50-EIA，大多数（138个中的132个）血清前样品未显示中和活性。接种疫苗后的8至12周，仍有17名儿童为阴性，但2.5岁后仅剩下7名儿童，血清转化率为125，为132（95％）。在同一时期，中和几何平均滴度从3.6升至9.9。通过间接ELISA，在138个血清前样品中发现128个阴性。 8名儿童的早期和晚期疫苗接种血清均为ELISA阴性，导致120的血清转化率为128（94％）。两种方法只有两个孩子保持血清阴性。疫苗接种后后期的血清样本中有7份结果不一致，反映了两种方法之间95％的相关性。由于与副流感病毒具有交叉反应性，因此ELISA的特异性较差，但与N50-EIA相比，其灵敏度更高。

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