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首页> 外文期刊>Journal of Clinical Microbiology >Monoclonal antibody to native P39 protein from Borrelia burgdorferi.
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Monoclonal antibody to native P39 protein from Borrelia burgdorferi.

机译：伯氏疏螺旋体中天然P39蛋白的单克隆抗体。

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摘要

We have produced, by using a sonicate of Borrelia burgdorferi, a monoclonal antibody (MAb), NYSP39H, that is specific for the P39 protein band. This MAb reacted with 13 isolates of B. burgdorferi but not with eight different spirochetes (four borrelias, two leptospiras, and two treponemas). Surface labeling of B. burgdorferi with biotin and subsequent treatment with Nonidet P-40 showed that P39 was not biotinylated but was extracted with Nonidet P-40, indicating that it is present within the outer membrane, but not on the surface of the spirochete. Immunoelectron microscopy revealed the immunogold probe primarily at the cytoplasmic membrane region of the spirochete. The MAb detected B. burgdorferi in the indirect fluorescent-antibody test only when the spirochetes from a culture or in a tick homogenate were fixed with polylysine and not with acetone. NYSP39H appears to be an appropriate probe for use in the specific detection of B. burgdorferi.
机译：我们通过使用博氏疏螺旋体的超声产生了一种单克隆抗体（MAb）NYSP39H，该抗体对P39蛋白条带具有特异性。该单克隆抗体与13株伯氏疏螺旋体反应，但不与8种不同的螺旋体反应（4种疏螺旋体，2种钩端螺旋体和2种螺旋体）。用生物素对B. burgdorferi进行表面标记，然后用Nonidet P-40处理，表明P39没有被生物素化，但被Nonidet P-40提取，表明它存在于外膜内，但不在螺旋体表面。免疫电子显微镜检查显示，免疫金探针主要位于螺旋体的胞质膜区域。仅当将培养物或壁虱匀浆中的螺旋体用聚赖氨酸固定而不用丙酮固定时，MAb才在间接荧光抗体测试中检测到伯氏疏螺旋体。 NYSP39H似乎是用于伯氏疏螺旋体特异性检测的合适探针。

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