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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Bovine Herpesvirus 1 Glycoprotein E Antibodies in Individual Milk Samples by Enzyme-Linked Immunosorbent Assays
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Detection of Bovine Herpesvirus 1 Glycoprotein E Antibodies in Individual Milk Samples by Enzyme-Linked Immunosorbent Assays

机译：酶联免疫吸附法检测单个牛奶样品中的牛疱疹病毒1糖蛋白E抗体

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摘要

The purpose of this study was to determine whether individual milk samples can replace serum samples for the detection of bovine herpesvirus 1 (BHV1) glycoprotein E (gE)-specific antibodies. Serum and milk samples were collected at the same time from cattle in BHV1-free herds, cattle in unvaccinated herds, and cattle in herds that were vaccinated twice with a BHV1 marker vaccine. The samples were tested in two gE enzyme-linked immunosorbent assay (ELISA) systems. In comparison to serum, the results showed that the gE-blocking ELISA was highly sensitive for testing milk samples (0.96). In contrast, the gE ELISA was less sensitive (0.79). The specificities of the gE-blocking ELISA and the gE ELISA for testing milk samples were very high (1.00 and 0.99, respectively). The presented results indicate that individual milk samples, which can be collected relatively easily and inexpensively, can be used instead of individual serum samples in the gE-blocking ELISA for the screening of cattle for BHV1 gE antibodies.
机译：这项研究的目的是确定单个牛奶样品是否可以代替血清样品以检测牛疱疹病毒1（BHV1）糖蛋白E（gE）特异性抗体。同时从不含BHV1的牛群，未接种牛群的牛和用BHV1标记疫苗两次接种牛群的牛中同时采集血清和牛奶样品。在两个gE酶联免疫吸附测定（ELISA）系统中测试了样品。与血清相比，结果表明，gE阻断ELISA对检测牛奶样品高度敏感（0.96）。相反，gE ELISA的敏感性较低（0.79）。 gE阻断ELISA和gE ELISA用于检测牛奶样品的特异性非常高（分别为1.00和0.99）。提出的结果表明，可以在gE阻断ELISA中使用可以相对容易且便宜地收集的单个牛奶样品代替单个血清样品，以筛选牛的BHV1 gE抗体。

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