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首页> 外文期刊>Journal of Clinical Microbiology >Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study
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Use of BACTEC MGIT 960 for Recovery of Mycobacteria from Clinical Specimens: Multicenter Study

机译：BACTEC MGIT 960从临床标本中恢复分枝杆菌的用途：多中心研究

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The BACTEC MGIT 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Its performance was compared to those of the radiometric BACTEC 460 instrument and egg-based Lowenstein-Jensen medium. An identical volume of sample was inoculated in different media, and incubation was carried out for 6 weeks with the automatic systems and for 8 weeks on solid media. A total of 2,567 specimens obtained from 1,631 patients were cultured in parallel. Mycobacteria belonging to nine different taxa were isolated by at least one of the culture systems, with 75% of them being represented byMycobacterium tuberculosis complex. The best yield was obtained with the BACTEC 460 system, with 201 isolates, in comparison with 190 isolates with the BACTEC MGIT 960 system and 168 isolates with Lowenstein-Jensen medium. A similar but not significant difference was obtained when the most-represented organisms, the M. tuberculosis complex, Mycobacterium xenopi, and theMycobacterium avium complex, were analyzed separately and when combinations of a solid medium with the BACTEC MGIT 960 system and with the BACTEC 460 system were considered. The shortest times to detection were obtained with the BACTEC MGIT 960 system (13.3 days); 1.5 days earlier than that with the BACTEC 460 system (14.8 days) and 12 days earlier than that with Lowenstein-Jensen medium (25.6 days). The BACTEC MGIT 960 system had a contamination rate of 10.0%, intermediate between those of the radiometric system (3.7%) and the egg-based medium (17.0%). We conclude, therefore, that the BACTEC MGIT 960 system is a fully automated, nonradiometric instrument that is suitable for the detection of growth of tuberculous and other mycobacterial species and that is characterized by detection times that are even shorter than that of the “gold standard,” the BACTEC 460 system. The contamination rate was higher than that for the radiometric BACTEC 460 system and needs to be improved.
机译：BACTEC MGIT 960仪器是一种全自动系统，该系统利用氧气传感器的荧光来检测培养物中分枝杆菌的生长。将其性能与辐射BACTEC 460仪器和蛋基Lowenstein-Jensen培养基的性能进行了比较。将相同体积的样品接种到不同的培养基中，并用自动系统孵育6周，在固体培养基上孵育8周。平行培养了从1,631名患者获得的2,567个标本。至少有一个培养系统分离了属于9个不同类群的分枝杆菌，其中75％的细菌是结核分枝杆菌。与使用BACTEC MGIT 960系统的190个菌株和使用Lowenstein-Jensen培养基的168个菌株相比，使用BACTEC 460系统可获得201个菌株的最佳产量。当最具代表性的生物 M时，获得了相似但不明显的差异。当将固体培养基与BACTEC MGIT 960系统和BACTEC组合使用时，分别分析了结核病复合物，异种分枝杆菌和鸟分枝杆菌复合物。考虑使用460系统。使用BACTEC MGIT 960系统（13.3天）可获得最短的检测时间。比使用BACTEC 460系统的时间（14.8天）提前1.5天，比使用Lowenstein-Jensen介质的时间（25.6天）提前12天。 BACTEC MGIT 960系统的污染率为10.0％，介于放射系统（3.7％）和蛋基培养基（17.0％）之间。因此，我们得出结论，BACTEC MGIT 960系统是一种全自动的非辐射仪器，适用于检测结核菌和其他分枝杆菌菌种的生长，并且其检测时间甚至比“金标准”的检测时间还要短。 ”，即BACTEC 460系统。污染率高于辐射BACTEC 460系统，需要改进。

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