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首页> 外文期刊>Journal of Clinical Microbiology >CDC Group IV c-2: a New RalstoniaSpecies Close to Ralstonia eutropha
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CDC Group IV c-2: a New RalstoniaSpecies Close to Ralstonia eutropha

机译:疾病预防控制中心IV- 2组:一种新的雷氏菌属,接近富营养的雷氏菌

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CDC group IV c-2, an environmental gram-negative bacillus recently proposed for inclusion in the genus Ralstonia, has been isolated in several human infections. Biochemical characterization and 16S ribosomal DNA (rDNA) sequencing with phylogenetic analysis were used to characterize eight clinical isolates and four type strains. Other typing tools, such as pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) analysis, were also used. PFGE typing of clinical isolates was unsuccessful because the DNA was degraded, and RAPD analysis was poorly discriminatory. In contrast, the type strains were clearly distinguished with both PFGE and RAPD analysis. All of the 16S rDNA sequences were identical. Comparison of the 16S rDNA sequences to the GenBank sequences showed that they were consistent with CDC group IV c-2 belonging to the genusRalstonia. The closest matches were obtained withRalstonia eutropha. However, four differences in 32 biochemical tests separated R. eutropha from CDC group IV c-2, which suggests that CDC group IV c-2 is a new species of the genusRalstonia.
机译:CDC IV c-2组是一种环境革兰氏阴性芽孢杆菌,最近被提议纳入属,已在几种人类感染中分离出来。生化特征和16S核糖体DNA(rDNA)测序与系统发育分析用于表征八种临床分离株和四种类型菌株。还使用了其他打字工具,例如脉冲场凝胶电泳(PFGE)和随机扩增多态性DNA(RAPD)分析。临床分离株的PFGE分型不成功,因为DNA降解,并且RAPD分析的鉴别性差。相反,通过PFGE和RAPD分析可以清楚地区分类型菌株。所有的16S rDNA序列都是相同的。 16S rDNA序列与GenBank序列的比较表明,它们与隶属于 Ralstonia 的CDC IV c-2组一致。最接近的匹配是 Ralstonia eutropha 。但是,在32个生化测试中有四个差异将 R分开。 CDC IV c-2组的富营养化,表明CDC IV c-2组是 Ralstonia 属的一个新物种。

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