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首页> 外文期刊>Journal of Clinical Microbiology >HEp-2 cell adherence patterns, serotyping, and DNA analysis of Escherichia coli isolates from eight patients with AIDS and chronic diarrhea.
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HEp-2 cell adherence patterns, serotyping, and DNA analysis of Escherichia coli isolates from eight patients with AIDS and chronic diarrhea.

机译：八名艾滋病和慢性腹泻患者的大肠杆菌分离株的HEp-2细胞粘附模式，血清分型和DNA分析。

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摘要

Three morphologic patterns of interaction between bacteria and enterocytes have been observed in colonic biopsy specimens from AIDS patients with chronic diarrhea in the United States. The DNA encoding virulence factors and the HEp-2 cell adherence patterns of Escherichia coli strains isolated from the stools of eight symptomatic AIDS patients were compared with those of five control strains with known adherence patterns. One clinical isolate from a patient with attaching-and-effacing enteropathy displayed the localized adherence attaching-and-effacing pattern typical of enteropathogenic E. coli on HEp-2 cells, five isolates displayed the "stacked-brick" aggregative adherence pattern typical of enteroaggregative E. coli strains, and one isolate showed the pattern characteristic of diffusely adherent E. coli. One patient's isolate displayed features of all three patterns. No clinical isolate hybridized with standard probes for enteropathogenic, enteroaggregative, diffusely adherent, enterotoxigenic, and enteroinvasive E. coli strains. Thus, isolates from symptomatic AIDS patients in the United States can display the same interactive patterns with HEp-2 cells as the agents of pediatric or traveler's diarrhea, but lack their typical virulence factors.
机译：在美国患有慢性腹泻的AIDS患者的结肠活检标本中，观察到了细菌和肠上皮细胞相互作用的三种形态学模式。从八名有症状的艾滋病患者的粪便中分离的大肠杆菌菌株的编码毒力因子的DNA和HEp-2细胞的粘附模式与已知粘附模式的五种对照菌株进行了比较。一名患有附着和面对肠病的患者的临床分离株显示出典型的肠道致病性大肠杆菌在HEp-2细胞上的局部附着和附着模式，五株分离株表现出典型的肠聚集性“堆积砖”聚集粘附模式大肠杆菌菌株和一种分离株表现出弥散性粘附的大肠杆菌的模式特征。一名患者的隔离株显示出所有三种模式的特征。没有与标准探针杂交的临床分离株可用于肠致病性，肠聚集性，弥散性粘附，产肠毒素和肠侵害性大肠杆菌菌株。因此，在美国，有症状的艾滋病患者的分离株可以与小儿或旅行者腹泻的病原体表现出与HEp-2细胞相同的相互作用模式，但缺乏典型的毒力因子。

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