Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures.

P M Small; N B McClenny; S P Singh; G K Schoolnik; L S Tompkins; P A Mickelsen

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Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures.

机译：结核分枝杆菌的分子菌株分型，以确认在分枝杆菌学实验室中的交叉污染，并修改了程序以最大程度地减少假阳性培养的发生。

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Molecular strain typing by restriction fragment length polymorphism analysis was used to demonstrate that two clusters of Mycobacterium tuberculosis cultures involving six patients resulted from cross-contamination in the mycobacteriology laboratory. Contaminated cultures were processed by the decontamination procedure and were read on the BACTEC instrument following acid-fast bacillus smear-positive specimens from patients with active tuberculosis. Investigation of these episodes suggested opportunities for modification of laboratory procedures to minimize cross-contamination and confirmed the adverse medical and public health consequences of false-positive cultures. Strain-typing results were used in decisions regarding patient care, including the curtailment of unnecessary treatment in one patient. Molecular strain typing appears to be a valuable means of identifying false-positive cultures of M. tuberculosis in selected settings.

机译：通过限制性片段长度多态性分析对分子菌株进行分型，证明分枝杆菌实验室中的交叉污染导致了两例结核分枝杆菌培养物的两个簇，涉及六名患者。受污染的培养物经过去污染程序处理，并在BACTEC仪器上从活动性肺结核患者的耐酸杆菌涂片阳性标本中读取。对这些事件的调查表明，有机会修改实验室程序，以最大程度地减少交叉污染，并证实假阳性文化对医学和公共卫生的不利影响。劳力分型结果用于有关患者护理的决策中，包括减少一名患者不必要的治疗。分子菌株分型似乎是鉴定选定环境中结核分枝杆菌假阳性培养物的有价值的手段。

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《Journal of Clinical Microbiology》 |1993年第7期|共6页
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P M Small; N B McClenny; S P Singh; G K Schoolnik; L S Tompkins; P A Mickelsen;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Mycobacterium tuberculosis typing: usefulness of DRE-PCR to confirm cross-contamination in the mycobacteriology laboratory of a general reference hospital for AIDS. [J] . Filho LA, Kritski AL, Salles CL, The international journal of tuberculosis and lung disease: the official journal of the International Union against Tuberculosis and Lung Disease . 2002,第2期

机译：结核分枝杆菌分型：DRE-PCR在确认艾滋病综合参考医院的分枝杆菌实验室中确认交叉污染的有用性。
2. Recognizing laboratory cross-contamination: Two false-positive cultures of mycobacterium tuberculosis - Oklahoma, 2011 [J] . JohnsonM.G., LindseyP.H., HarveyC.F., Chest: The Journal of Circulation, Respiration and Related Systems . 2013,第1期

机译：认识实验室交叉污染：结核分枝杆菌的两种假阳性培养物-俄克拉荷马州，2011年
3. False-positive results from cultures of Mycobacterium tuberculosis due to laboratory cross-contamination confirmed by restriction fragment length polymorphism. [J] . J Bauer, V O Thomsen, S Poulsen, Journal of Clinical Microbiology . 1997,第4期

机译：限制性片段长度多态性证实了由于实验室交叉污染而导致的结核分枝杆菌培养物的假阳性结果。
4. Molecular typing of Mycobacterium avium subsp. paratuberculosis strains from different hosts and regions [C] . Sevilla I, Singh SV, Garrido JM, International Colloquium on Paratuberculosis . 2007

机译：分子型分子分枝杆菌亚空间。来自不同主持人和地区的副植物菌菌株
5. Type I Interferon Induction by Diverse Strains of the Mycobacterium Tuberculosis Complex. [D] . Wiens, Kirsten E. 2017

机译：不同菌株结核分枝杆菌复合物的I型干扰素诱导。
6. Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures. [O] . P M Small, N B McClenny, S P Singh, 1993

机译：结核分枝杆菌的分子菌株分型以确认在分枝杆菌学实验室中的交叉污染并修改了程序以最大程度地减少假阳性培养的发生。
7. Optimized molecular resolution of cross-contamination alerts in clinical mycobacteriology laboratories [O] . 2008

机译：临床分枝杆菌实验室中优化的交叉污染警报分子分辨率

Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures.

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