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首页> 外文期刊>Journal of Clinical Microbiology >Molecular epidemiology of a fast-food restaurant-associated outbreak of Escherichia coli O157:H7 in Washington State.
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Molecular epidemiology of a fast-food restaurant-associated outbreak of Escherichia coli O157:H7 in Washington State.

机译：华盛顿州快餐店相关大肠杆菌O157：H7爆发的分子流行病学研究。

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We studied the molecular epidemiology of the recent fast-food restaurant chain-associated Escherichia coli O157:H7 outbreak in Washington State. Genomic DNAs prepared from strains isolated from 433 patients were probed with radiolabelled Shiga-like toxin (SLT) I and SLT II genes and bacteriophage lambda DNA and were subsequently analyzed for their restriction fragment length polymorphism (RFLP) patterns. The SLT RFLP and lambda RFLP profiles of an E. coli O157:H7 strain isolated from the incriminated beef and prototype patient were compared with those of the patient isolates for determination of the concordance between patterns. Of the 377 patients with primary and secondary cases of infection epidemiologically linked to the outbreak, isolates from 367 (97.3%) of the patients displayed SLT RFLP and lambda RFLP profiles identical to those of the outbreak strains. Isolates from 10 of the 377 (2.6%) patients possessed SLT RFLP and lambda RFLP profiles different from those of the outbreak strains, and the patients from whom those isolates were obtained were subsequently characterized as having non-outbreak-related infections. The E. coli O157:H7 strains isolated from 31 of 44 (70.4%) patients who were epidemiologically excluded from the outbreak were linked to the outbreak by RFLP typing. Our results indicate that SLT RFLP and lambda RFLP analyses are stable and sensitive methods, and when they are used in conjunction with an epidemiological investigation they could result in an earlier recognition of outbreaks and their sources, hence prompting measures to prevent the continued transmission of E. coli O157:H7.
机译：我们研究了华盛顿州最近与快餐店连锁的大肠杆菌O157：H7爆发的分子流行病学。用放射标记的志贺样毒素（SLT）I和SLT II基因以及噬菌体λDNA探测从433名患者中分离出的菌株制备的基因组DNA，随后对其限制性片段长度多态性（RFLP）模式进行分析。从确诊的牛肉和原型患者中分离的大肠杆菌O157：H7菌株的SLT RFLP和lambda RFLP图谱与患者分离株的SLT RFLP和lambda RFLP图谱进行了比较，以确定模式之间的一致性。在流行病学上与暴发流行有关的377例原发和继发感染病例中，有367例（97.3％）患者分离出的SLT RFLP和lambda RFLP谱与暴发菌株相同。 377名患者中有10名（2.6％）的分离株具有与暴发菌株不同的SLT RFLP和lambda RFLP图谱，随后从中获得这些分离株的患者被定性为非暴发相关感染。从流行病学上被排除在暴发流行之外的44例患者中的31例（70.4％）分离出的O157：H7大肠杆菌菌株通过RFLP分型与暴发相关。我们的结果表明SLT RFLP和lambda RFLP分析是稳定且敏感的方法，当将它们与流行病学调查结合使用时，它们可能会导致对疫情及其来源的更早认识，从而促使采取措施防止E继续传播大肠杆菌O157：H7。

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