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首页> 外文期刊>Journal of Clinical Microbiology >Small Subunit Ribosomal DNA Sequence ShowsParacoccidioides brasiliensis Closely Related toBlastomyces dermatitidis
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Small Subunit Ribosomal DNA Sequence ShowsParacoccidioides brasiliensis Closely Related toBlastomyces dermatitidis

机译：小亚基核糖体DNA序列显示巴西乳杆菌（Paracoccidioides brasiliensis）与皮肤芽孢杆菌（Blastomyces dermatitidis）密切相关

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The similarities of paracoccidioidomycosis and blastomycosis are highly suggestive of a close relation of the two etiological agents. Whereas the agent of the first disease is exclusively endemic in Latin America, the agent of the latter one is endemic in North America and Africa. In symptomatic travelers visiting both areas of endemicity, differentiation of the diseases might be impossible, even though therapy and prognosis for these two diseases differ significantly. In order to identify differences in the 18S rRNA gene (rDNA) for use as molecular diagnostic tools, we sequenced this gene from five isolates of Paracoccidioides brasiliensis and compared them to known sequences of other fungi. Neighbor-joining, maximum parsimony, and maximum likelihood analyses and, finally, the Kishino-Hasegawa test revealed that P. brasiliensis, Blastomyces dermatitidis, and Emmonsia parva are more closely related than Histoplasma capsulatum and B. dermatitidis, whose teleomorphic forms belong to one genus,Ajellomyces. In accordance with the work of other investigators who have used internal transcribed spacer and large subunit rDNA sequences, our small subunit rDNA data show that the dimorphic fungus P. brasiliensis must be grouped within the order Onygenales and is closely related to members of the family Onygenaceae. There are hints in the molecular phylogenetic analysis that the family Onygenaceae might be further divided into two families. The subgroup that includes P. brasiliensis comprises all zoopathogenic species. The differences in the 18S rDNAs appear to be too small to allow species identification of the members of the family Onygenaceae pathogenic for humans by use of target sequences within this gene.
机译：副球菌和芽孢杆菌病的相似性高度暗示了这两种病原体的密切关系。第一种疾病的病原体仅在拉丁美洲流行，而第二种疾病的病原体在北美和非洲是地方病。在有症状的旅行者中，访问这两个地方性流行地区，即使对这两种疾病的治疗和预后有显着差异，也可能无法区分疾病。为了鉴定用作分子诊断工具的18S rRNA基因（rDNA）的差异，我们从五种巴西对虾（Paracoccidioides brasiliensis）分离物中对该基因进行了测序，并将其与其他真菌的已知序列进行了比较。邻域连接，最大简约性和最大似然分析以及最后的长野gawa野检验表明， P。 Brasiliensis ， Blastomyces dermatitidis 和 Emmonsia parva 比荚膜支原体和 B密切相关。皮肤亚种，其亚同形体属于一个属， Ajellomyces 。根据其他使用内部转录间隔区和大亚基rDNA序列的研究者的工作，我们的小亚基rDNA数据表明，双态真菌 P。 brasiliensis 必须归类为 Onygenales ，并且与 Onygenaceae 家族成员密切相关。在分子系统发育分析中暗示，On科（emygenaceae）可能进一步分为两个科。包含 P的子组。巴西利亚包含所有动物致病物种。 18S rDNA的差异似乎太小，无法通过使用该基因内的靶序列来鉴定对人类有致病力的Onygenaceae 成员。

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