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首页> 外文期刊>Journal of Clinical Microbiology >Identification of Strains ofAlcaligenes and Agrobacterium by a Polyphasic Approach
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Identification of Strains ofAlcaligenes and Agrobacterium by a Polyphasic Approach

机译：多相法鉴定产碱杆菌和农杆菌菌株

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The number of stable discriminant biochemical characters is limited in the genera Alcaligenes andAgrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. Moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, Alcaligenes xylosoxidans was transferred to the genus Achromobacter in 1998. Twenty-five strains of Achromobacter xylosoxidans, three strains of an Agrobacterium sp., five strains of anAlcaligenes sp., and four unnamed strains belonging to the Centers for Disease Control and Prevention group IVc-2 were examined. These strains were characterized by conventional tests, including biochemical tests. The assimilation of 99 carbohydrates, organic acids, and amino acids was studied by using Biotype-100 strips, and rRNA gene restriction patterns were obtained with the automated Riboprinter microbial characterization system after cleavage of total DNA with EcoRI or PstI restriction endonuclease. This polyphasic approach allowed the two subspecies ofA. xylosoxidans to be clearly separated. Relationships between five strains and the Ralstonia paucula type strain were demonstrated. Likewise, three strains were found to be related to the Ochrobactrum anthropi type strain. We showed that substrate assimilation tests and automated ribotyping provide a simple, rapid, and reliable means of identifying A. xylosoxidans subspecies and that these two methods can be used as alternative methods to characterize unidentified strains rapidly when discriminant biochemical characters are missing.
机译：稳定的判别生化特征的数目在产碱杆菌属和农杆菌属中受到限制，因此很难通过常规试验将它们区分。而且，基因组研究最近极大地改变了这些属的名称。例如，1998年将产碱木聚糖氧化酶转移到无色杆菌属。25株 Achromobacter xylosoxidans 菌株是三株检查了土壤杆菌属，五种产碱杆菌属的菌株和疾病预防控制中心IVc-2组的四株未命名菌株。这些菌株通过常规测试包括生化测试进行了表征。用Biotype-100试纸研究了99种碳水化合物，有机酸和氨基酸的同化作用，并用 Eco RI切割总DNA后，利用自动Riboprinter微生物鉴定系统获得了rRNA基因的限制性酶切图谱。或 Pst I限制性核酸内切酶。这种多相方法允许 A的两个亚种。木糖氧化酶要明确分开。证实了五株菌株与 Ralstonia paucula 型菌株之间的关系。同样，发现了三个与人类em骨人型（Ochrobactrum anthropi ）菌株有关的菌株。我们证明了底物同化试验和自动核糖分型法提供了一种简单，快速和可靠的鉴定 A的方法。木二氧化物亚种，这两种方法可作为替代方法，以在缺少判别性生化特征时快速鉴定未鉴定的菌株。

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