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首页> 外文期刊>Journal of Clinical Microbiology >Integrated Human Papillomavirus Type 16 Is Frequently Found in Cervical Cancer Precursors as Demonstrated by a Novel Quantitative Real-Time PCR Technique
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Integrated Human Papillomavirus Type 16 Is Frequently Found in Cervical Cancer Precursors as Demonstrated by a Novel Quantitative Real-Time PCR Technique

机译：新型实时荧光定量PCR技术在宫颈癌前体中经常发现16型整合型人乳头瘤病毒

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In contrast to cervical cancer, integration of human papillomavirus (HPV) DNA into the host genome has been considered a rare event in cancer precursor lesions (cervical intraepithelial neoplasia [CIN]). With our new real-time PCR method, we demonstrated that integrated HPV type 16 (HPV16) is already present in CIN lesions. The physical state of HPV16 and the viral load were simultaneously detected. A unique region of the E2 open reading frame (ORF) that is most often deleted during HPV16 integration is targeted by one set of PCR primers and a probe, and another set targets the E6 ORF. In episomal form, both targets should be equivalent, while in integrated form, the copy numbers of E2 would be less than those of E6. The method was tested with DNAs from 31 cervical lesions (non-CIN to CINIII) from 24 women prospectively followed up for 10 years. This report presents viral load and integration results from the largest series of CIN lesions described to date. Only one sample contained exclusively episomal HPV16 DNA, and this lesion regressed spontaneously. Samples from another patient, with only integrated HPV16, rapidly progressed from CINI to CINIII in 2 years. In all other patients, episomal and integrated forms of HPV16 DNA were found to coexist. Rapid progression of the CIN lesions was closely associated with a heavy load of integrated HPV16. Thus, the method described here is a very sensitive tool with which to assess the physical state of HPV, which is useful in predicting disease progression.
机译：与宫颈癌相反，人类乳头瘤病毒（HPV）DNA整合入宿主基因组被认为是癌症前体病变（宫颈上皮内瘤变[CIN]）中的罕见事件。通过我们的新的实时PCR方法，我们证明了CIN病变中已经存在集成的16型HPV（HPV16）。同时检测HPV16的物理状态和病毒载量。 E2开放阅读框（ORF）的唯一区域（在HPV16整合过程中最常被删除）以一组PCR引物和一个探针为目标，而另一组以E6 ORF为目标。在游离形式下，两个靶标应相等，而在整合形式下，E2的拷贝数应小于E6的拷贝数。该方法用来自24位女性的31个宫颈病变（非CIN至CINIII）的DNA进行了测试，预期随访了10年。该报告介绍了迄今为止描述的最大系列的CIN病变的病毒载量和整合结果。仅一个样品仅包含游离型HPV16 DNA，并且该病变自发消退。另一名仅带有HPV16整合型患者的样本在2年内从CINI迅速发展为CINIII。在所有其他患者中，发现游离和整合形式的HPV16 DNA共存。 CIN病变的快速发展与整合的HPV16的沉重负荷密切相关。因此，此处描述的方法是一种非常敏感的工具，可用来评估HPV的物理状态，这可用于预测疾病的进展。

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