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首页> 外文期刊>Journal of Clinical Microbiology >Aspergillus Hyphae in Infected Tissue: Evidence of Physiologic Adaptation and Effect on Culture Recovery
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Aspergillus Hyphae in Infected Tissue: Evidence of Physiologic Adaptation and Effect on Culture Recovery

机译：感染组织中的曲霉菌丝：生理适应性的证据及其对培养物回收的影响

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Microbiologic cultures of fungi are routinely incubated at ambient temperatures in room air, and the rate of recovery of Aspergillus species from clinical specimens is poor. Failure of current culture methods to mimic the physiologic temperature and low-oxygen environment found in hypha-laden infected tissue may underlie this poor recovery. Experiments were performed to compare the recovery of Aspergillus spp. incubated at 35°C in 6% O2-10% CO2 with that at 25°C in room air. The samples tested included Aspergillus-infected tissue specimens from a dog model and human autopsies, experimental anaerobically stressed Aspergillus inocula, and 10,062 consecutive clinical specimens. Culture at 35°C in 6% O2-10% CO2 significantly enhanced the recovery of Aspergillus spp. from the infected autopsy tissue samples. Incubation at 35°C alone resulted in approximately 10-fold-improved culture recovery from the experimentally stressed hyphae, and the 6% O2-10% CO2 atmosphere independently favored growth under temperature-matched conditions. Finally, incubation at 35°C (in room air) improved the overall recovery of Aspergillus spp. from clinical specimens by 31%. Culture at 35°C in a microaerobic atmosphere significantly enhances the recovery of Aspergillus spp. from various sources. Aspergillus hyphae growing in infected tissue appear to be adapted to the physiologic temperature and hypoxic milieu.
机译：真菌的微生物培养物通常在室温下在室内空气中孵育，并且从临床标本中回收曲霉物种的速度很差。当前的培养方法无法模仿在富含菌丝的感染组织中发现的生理温度和低氧环境，可能是这种不良恢复的基础。进行了实验以比较曲霉菌的回收率。在5％O 2 -10％CO 2 和35°C的室内空气中于35°C孵育。测试的样品包括来自狗模型和人的尸体的曲霉感染的组织标本，实验厌氧应激的曲霉接种物以及10,062个连续的临床标本。在35°C的6％O 2 -10％CO 2 中培养可显着提高曲霉 spp的回收率。从被感染的尸检组织样本中提取。单独在35°C下温育可从实验压力菌丝中恢复大约10倍的培养物回收率，并且独立地支持6％O 2 -10％CO 2 气氛在温度匹配的条件下生长。最后，在35°C（在室内空气中）孵育可改善曲霉 spp的总体回收率。从临床标本中抽取31％。在微需氧气氛下于35°C培养可显着提高曲霉 spp的恢复。来自各种来源。在感染组织中生长的曲霉菌丝似乎适应了生理温度和低氧环境。

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