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首页> 外文期刊>Journal of Clinical Microbiology >Utility of Mycobacterial Interspersed Repetitive Unit Typing for Differentiating Multidrug-Resistant Mycobacterium tuberculosis Isolates of the Beijing Family
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Utility of Mycobacterial Interspersed Repetitive Unit Typing for Differentiating Multidrug-Resistant Mycobacterium tuberculosis Isolates of the Beijing Family

机译：分枝杆菌散布的重复单元分型在区​​分北京家庭耐多药结核分枝杆菌中的应用

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Mycobacterial interspersed repetitive unit (MIRU) typing has been found to allow rapid, reliable, high-throughput genotyping of Mycobacterium tuberculosis and may represent a feasible approach to study global M. tuberculosis molecular epidemiology. To evaluate the use of MIRU typing in discriminating drug-resistant M. tuberculosis strains of the Beijing genotype family, 102 multidrug-resistant (MDR) clinical isolates and 253 randomly selected non-MDR isolates collected from 2000 to 2003 in Hong Kong were subjected to 12-locus MIRU typing, spoligotyping, and IS6110 restriction fragment length polymorphism (RFLP) typing. Spoligotyping showed that 243 (68.5%) of 355 isolates belonged to Beijing family genotype. MIRU typing showed lower discrimination in differentiating between the Beijing family strains (Hunter-Gaston discriminative index [HGI] of 0.8827) compared with the IS6110 RFLP method (HGI = 0.9979). For non-Beijing strains, MIRU typing provided discrimination (HGI = 0.9929) comparable to that of the RFLP method (HGI = 0.9961). There was no remarkable difference in discrimination power between the two methods in differentiating both within and between MDR and non-MDR strains of M. tuberculosis. Dendrograms constructed with the MIRU typing data showed a clear segregation between the Beijing and non-Beijing genotype. Addition of RFLP to MIRU typing offered a higher discrimination ability (92.6%) than did addition of MIRU typing to RFLP (40.0%). This supported the potential use of this method to analyze the global genetic diversity of MDR M. tuberculosis strains that may be at different levels of evolutionary divergence.
机译：已经发现分枝杆菌散布的重复单位（MIRU）分型可以快速，可靠，高通量地对结核分枝杆菌进行基因分型，并且可能代表研究整体 M的可行方法。结核分子流行病学。为了评估MIRU分型在区分耐药性 M中的用途。 2000年至2003年在香港收集的北京基因型家族的结核病菌株，102株耐多药（MDR）临床分离株和253随机选择的非MDR分离株进行了12位MIRU分型，血吸虫分型和IS 6110 限制性片段长度多态性（RFLP）分型。 Spoligotyping分析表明，在355个分离株中，有243个（68.5％）属于北京家族基因型。与IS 6110 RFLP方法（HGI = 0.9979）相比，MIRU分型显示出对北京家族菌株的区分（Hunter-Gaston判别指数[HGI]为0.8827）的区分度较低。对于非北京菌株，MIRU分型可提供与RFLP方法（HGI = 0.9961）相当的区分度（HGI = 0.9929）。两种方法在区分 M的MDR菌株与非MDR菌株之间以及之间的区分能力方面没有显着差异。结核病。用MIRU分型数据构建的树状图显示出北京和非北京基因型之间的明显隔离。与在RFLP中添加MIRU类型（40.0％）相比，在MIRU类型中添加RFLP提供了更高的辨别能力（92.6％）。这支持了该方法可能用于分析耐多药M的全球遗传多样性。结核菌株可能处于不同的进化分化水平。

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