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首页> 外文期刊>Journal of Clinical Microbiology >Variation of the Mycobacterium tuberculosis PE_PGRS33 Gene among Clinical Isolates
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Variation of the Mycobacterium tuberculosis PE_PGRS33 Gene among Clinical Isolates

机译：临床分离株之间结核分枝杆菌PE_PGRS33基因的变异

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PE_PGRS33, one of about 60 PE_PGRS genes in the Mycobacterium tuberculosis genome, encodes a surface-expressed protein that may be involved in the antigenic variation of M. tuberculosis strains and evasion of the host immune system. While genetic differences between the PE_PGRS33 genes of H37Rv and CDC1551 have been noted, genetic variation in this gene among clinical isolates has not been evaluated. In order to gain a better understanding of the genetic basis for the role of PE_PGRS in antigenic variation and evasion of the host immune system, we investigated the genetic diversity of the PE_PGRS33 gene among 123 clinical M. tuberculosis isolates from a population-based study, using PCR and DNA sequencing. The 123 isolates belonged to principal genetic groups 1, 2, and 3 and had IS6110 copy numbers ranging from 1 to 22. Eighty-four (68.3%) of the 123 isolates were found to have at least one sequence variation in the PE_PGRS33 gene, relative to that of H37Rv. Twenty-five different sequence variations were observed and included three insertions (ranging from 9 to 87 bp), nine deletions (ranging from 1 to 273 bp), one insertion-and-deletion event, and 12 single-nucleotide polymorphisms (six synonymous and six nonsynonymous). Analysis of the relationships among the different PE_PGRS33 gene sequence variations suggests that polymorphisms in the gene are shifting along evolutionary lineages. The observed genetic diversity of the PE_PGRS33 gene supports its role in antigenic variation and can serve as a basis for future investigations of the function of the PE_PGRS33 gene among clinical isolates.
机译：PE_PGRS33是结核分枝杆菌基因组中约60个PE_PGRS基因之一，它编码一种表面表达的蛋白质，可能与 M的抗原变异有关。结核菌株和宿主免疫系统的逃避。尽管已经注意到H37Rv和CDC1551的PE_PGRS33基因之间的遗传差异，但尚未评估该临床分离株之间该基因的遗传变异。为了更好地了解PE_PGRS在抗原变异和逃避宿主免疫系统中的作用的遗传基础，我们调查了123位临床 M中PE_PGRS33基因的遗传多样性。 PCR和DNA测序从一项基于人群的研究中分离出结核菌。 123株分离株属于主要基因组1、2和3，其IS 6110 拷贝数范围为1至22。发现123株分离株中有84（68.3％）个具有至少相对于H37Rv，PE_PGRS33基因有一个序列变异。观察到二十五个不同的序列变异，包括三个插入（从9到87 bp），九个缺失（从1到273 bp），一个插入和删除事件以及12个单核苷酸多态性（六个同义和六个非同义）。对不同的PE_PGRS33基因序列变异之间的关系的分析表明，基因中的多态性正沿着进化谱系转移。观察到的PE_PGRS33基因的遗传多样性支持其在抗原变异中的作用，并可作为将来对临床分离株中PE_PGRS33基因功能的研究的基础。

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