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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Bacteroides fragilis in clinical specimens by PCR.
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Detection of Bacteroides fragilis in clinical specimens by PCR.

机译：通过PCR检测临床标本中的脆弱拟杆菌。

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The direct detection of Bacteroides fragilis from clinical specimens was examined by using the PCR method for amplifying a specific fragment of the glutamine synthetase gene from B. fragilis. By this method, all five B. fragilis strains tested were detected, but DNAs from anaerobic bacteria of 24 other species tested, from aerobic bacteria of 12 species tested, and from human leukocytes were not amplified. Using the nested PCR method, we were able to detect as little as one bacterial cell or 100 fg of chromosomal DNA of B. fragilis. A total of 39 clinical specimens, which consisted of 19 bronchial aspirates, 10 percutaneous lung aspirates, 2 transtracheal aspirates, 6 pleural fluid specimens, and 2 pus specimens, were tested. All four culture-positive samples, of which two were bronchial aspirates, one was pleural fluid, and one was pus, were positive by PCR. Among 35 culture-negative samples, 2 bronchial aspirates were positive by PCR. One was from a patient whose two previous samples were positive by both culture and PCR. It had been submitted for culture several hours after collection, and clindamycin had been administered to the patient before collection of the specimen. The other bronchial aspirate positive by PCR was from a pneumonia patient who had also been administered clindamycin. We believe that B. fragilis was present in these two specimens but that either it was dead, it was below the level detectable by culture, or the process of anaerobic culture was unsuccessful. Thus, the PCR method may be considered useful for the sensitive and rapid detection of anaerobes in clinical specimens.
机译：通过使用PCR方法扩增来自脆弱芽孢杆菌的谷氨酰胺合成酶基因的特定片段，检查了从临床标本中直接鉴定脆弱拟杆菌的方法。通过这种方法，检测到所有五种脆弱的博德特氏菌菌株，但未扩增来自其他24种厌氧菌，12种需氧菌和人白细胞的DNA。使用巢式PCR方法，我们能够检测到少至一个细菌细胞或100 fg脆弱脆弱芽孢杆菌的染色体DNA。总共测试了39个临床标本，包括19个支气管抽吸物，10个经皮肺抽吸物，2个经气管抽吸物，6个胸水标本和2个脓液标本。通过PCR，所有四个培养阳性样品，其中两个是支气管抽吸物，一个是胸膜液，一个是脓液。在35个培养阴性样品中，有2个支气管抽吸物通过PCR呈阳性。一个来自患者，该患者的先前两个样本通过培养和PCR均为阳性。它已在收集后几个小时提交培养，在收集标本之前已向患者施用了克林霉素。 PCR另一支阳性的支气管抽吸物来自肺炎患者，该患者也曾接受克林霉素治疗。我们认为这两个标本中均存在脆弱的芽孢杆菌，但要么死亡，要么低于培养物可检测的水平，否则厌氧培养过程将失败。因此，PCR方法可被认为对临床标本中厌氧菌的灵敏和快速检测有用。

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