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首页> 外文期刊>Journal of Clinical Microbiology >Molecular Analysis of Mycobacterium avium Isolates by Using Pulsed-Field Gel Electrophoresis and PCR
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Molecular Analysis of Mycobacterium avium Isolates by Using Pulsed-Field Gel Electrophoresis and PCR

机译：鸟分枝杆菌分离物的脉冲场凝胶电泳和PCR分子分析

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摘要

Genetic relationships among 46 isolates of Mycobacterium avium recovered from 37 patients in a 2,500-bed hospital from 1993 to 1998 were assessed by pulsed-field gel electrophoresis (PFGE) and PCR amplification of genomic sequences located between the repetitive elements IS1245 and IS1311. Each technique enabled the identification of 27 to 32 different patterns among the 46 isolates, confirming that the genetic heterogeneity ofM. avium strains is high in a given community. Furthermore, this retrospective analysis of sporadic isolates allowed us (i) to suggest the existence of two remanent strains in our region, (ii) to raise the question of the possibility of nosocomial acquisition ofM. avium strains, and (iii) to document laboratory contamination. The methods applied in the present study were found to be useful for the typing of M. avium isolates. In general, both methods yielded similar results for both related and unrelated isolates. However, the isolates in five of the six PCR clusters were distributed among two to three PFGE patterns, suggesting that this PCR-based method may have limitations for the analysis of strains with low insertion sequence copy numbers or for resolution of extended epidemiologic relationships.
机译：通过脉冲场凝胶电泳（PFGE）和PCR重复序列之间的基因组序列扩增，评估了1993年至2,500张病床的37例患者中1993年从37例患者中回收的46株鸟分枝杆菌的遗传关系。元素IS 1245 和IS 1311 。每种技术都能在46个分离物中鉴定出27至32种不同的模式，从而证实了 M的遗传异质性。在一个特定的社区中，avium 菌株很高。此外，这种对零星分离株的回顾性分析使我们（i）建议在我们地区存在两个残留菌株，（ii）提出了医院内获得 M的可能性的问题。 avium 菌株，以及（iii）记录实验室污染。发现本研究中应用的方法对于 M的分型很有用。 avium 分离株。通常，两种方法对于相关和不相关的分离株均产生相似的结果。但是，六个PCR簇中的五个中的分离株分布在两到三个PFGE模式之间，这表明这种基于PCR的方法可能对于分析低插入序列拷贝数的菌株或解决扩展的流行病学关系具有局限性。

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