...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Molecular Markers Demonstrate that the First Described Multidrug-Resistant Mycobacterium bovisOutbreak Was Due to Mycobacterium tuberculosis
【24h】

Molecular Markers Demonstrate that the First Described Multidrug-Resistant Mycobacterium bovisOutbreak Was Due to Mycobacterium tuberculosis

机译：分子标记物证明第一个描述的耐多药分枝杆菌牛分枝杆菌的爆发是由于结核分枝杆菌引起的

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We genetically characterized multidrug-resistantMycobacterium tuberculosis complex strains which caused a nosocomial outbreak of tuberculosis affecting six human immunodeficiency virus (HIV)-positive patients and one HIV-negative staff member (E. Bouvet, E. Casalino, G. Mendoza-Sassi, S. Lariven, E. Vallée, M. Pernet, S. Gottot, and F. Vachon, AIDS 7:1453–1460, 1993). The strains showed all the phenotypic characteristics ofMycobacterium bovis. They presented a high copy number of IS6110, the spacers 40 to 43 in the direct repeat locus, and the mtp40 fragment. They lacked the G-A mutation at position 285 in the oxyR gene and the C-G mutation at position 169 in the pncA gene. These genetic characteristics revealed that these were dysgonic, slow-growingM. tuberculosis strains mimicking the M. bovisphenotype, probably as a consequence of cellular alterations associated with the multidrug resistance. Spoligotyping and IS6110restriction fragment length polymorphism (RFLP) analysis confirmed that the outbreak was due to a single strain. However, the IS6110 RFLP pattern of the strain isolated from the last patient, diagnosed three years after the index case, differed slightly from the patterns of the other six strains. A model of a possible genetic event is presented to explain this divergence. This study stresses the value of using several independent molecular markers to identify multidrug-resistant tubercle bacilli.
机译：我们对耐多药的结核分枝杆菌复杂菌株进行了遗传鉴定，这些菌株引起了医院内的结核病暴发，影响了6名人类免疫缺陷病毒（HIV）阳性患者和1名HIV阴性工作人员（E. Bouvet，E. Casalino ，G。Mendoza-Sassi，S。Lariven，E。Vallée，M。Pernet，S。Gottot和F. Vachon，艾滋病7：1453-1460，1993）。菌株表现出牛分枝杆菌的所有表型特征。他们展示了很高的IS 6110 拷贝数，直接重复基因座的40至43个间隔区以及 mtp40 片段。他们在 oxyR 基因的285位缺乏G-A突变，在 pncA 基因的169位缺乏C-G突变。这些遗传特征表明它们是发育不良的，生长缓慢的M。模仿 M的结核菌菌株。牛表型，可能是与多药耐药性相关的细胞改变的结果。 Spoligotyping和IS 6110 限制性片段长度多态性（RFLP）分析证实，暴发是由于单一菌株引起的。但是，从最后一例患者分离出的菌株的IS 6110 RFLP模式在索引病例后三年被诊断出，与其他六种菌株的模式略有不同。提出了可能的遗传事件模型来解释这种差异。这项研究强调使用几种独立的分子标记物鉴定耐多药结核杆菌的价值。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号