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首页> 外文期刊>Journal of Clinical Microbiology >Detection of Ebola Viral Antigen by Enzyme-Linked Immunosorbent Assay Using a Novel Monoclonal Antibody to Nucleoprotein
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Detection of Ebola Viral Antigen by Enzyme-Linked Immunosorbent Assay Using a Novel Monoclonal Antibody to Nucleoprotein

机译：新型的核蛋白单克隆抗体酶联免疫吸附法检测埃博拉病毒抗原。

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With the increase in international traffic, the risk of introducing rare but severe infectious diseases like Ebola hemorrhagic fever is increasing all over the world. However, the system for the diagnosis of Ebola virus infection is available in a limited number of countries. In the present study, we developed an Ebola virus antigen-detection enzyme-linked immunosorbent assay (ELISA) system using a novel monoclonal antibody (MAb) to the nucleoprotein (NP). This antibody recognized an epitope defined by a 26-amino-acid stretch near the C terminus of NP. In a sandwich ELISA system with the MAb, as little as 30 ng of purified recombinant NP (rNP) was detected. Although this MAb was prepared by immunization with rNP of subtype Zaire, it also reacted to the corresponding region of NP derived from the Reston and Sudan subtypes. These results suggest that our ELISA system should work with three of four Ebola subtypes. Furthermore, our ELISA system detected the NP in subtype Reston-infected monkey specimens, while the background level in noninfected specimens was very low, suggesting the usefulness of the ELISA for laboratory diagnosis with clinical specimens.
机译：随着国际流量的增加，在世界范围内引入罕见但严重的传染病（如埃博拉出血热）的风险也在增加。但是，在少数国家/地区可以使用诊断埃博拉病毒感染的系统。在本研究中，我们开发了一种使用针对核蛋白（NP）的新型单克隆抗体（MAb）的埃博拉病毒抗原检测酶联免疫吸附测定（ELISA）系统。该抗体识别由NP的C末端附近的26个氨基酸延伸限定的表位。在具有MAb的夹心ELISA系统中，检测到低至30 ng的纯化重组NP（rNP）。尽管该单克隆抗体是通过用扎伊尔亚型的rNP免疫制备的，但它也与衍生自Reston和Sudan亚型的NP的相应区域反应。这些结果表明我们的酶联免疫吸附测定系统应适用于四种埃博拉亚型中的三种。此外，我们的ELISA系统检测了Reston感染的亚型猴标本中的NP，而未感染的标本中的背景水平非常低，这表明ELISA在临床标本的实验室诊断中很有用。

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