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Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein

机译:抗原捕获酶联免疫吸附测定可特异性检测雷斯顿埃博拉病毒核蛋白

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Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.
机译:抗原捕获酶联免疫吸附测定(ELISA)是快速检测埃博拉病毒的最有用方法之一。我们先前使用单克隆抗体(MAb)3-3D开发了一种抗原捕获ELISA,该抗体不仅与扎伊尔埃博拉病毒(EBO-Z)的核蛋白(NP)反应,而且也与苏丹的NP(EBO-S)反应和Reston Ebola(EBO-R)病毒。在这项研究中,我们开发了使用两种新颖的单克隆抗体Res2-6C8和Res2-1D8特异性针对EBO-R NP的抗原捕获ELISA。 Res2-6C8和Res2-1D8在EBO-R NP的C端区域附近分别识别了由4和8个氨基酸残基组成的表位。使用这两种单克隆抗体的抗原捕获ELISA从感染EBO-R的食蟹猕猴中检测到了组织中的EBO-R NP。使用Res2-6C8和Res2-1D8的抗原捕获ELISA可用于快速检测EBO-R感染的食蟹猕猴中的NP。

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