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首页> 外文期刊>Japanese journal of infectious diseases >Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus.
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Marburgvirus nucleoprotein-capture enzyme-linked immunosorbent assay using monoclonal antibodies to recombinant nucleoprotein: detection of authentic Marburgvirus.

机译：使用重组核蛋白单克隆抗体的马尔堡病毒核蛋白捕获酶联免疫吸附试验：检测真正的马尔堡病毒。

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摘要

There have recently been large outbreaks of Marburg hemorrhagic fever (MHF) caused by Marburgvirus (MARV) in the Democratic Republic of Congo and Angola. The development of reliable diagnostic systems for MHF is urgently needed. An antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) using either of the two monoclonal antibodies (2A7 and 2H6) produced by immunizing mice with recombinant nucleoprotein of MARV was described (Journal of Medical Virology, 76, 111-118, 2005). In the present study, it was revealed that the Ag-capture ELISA specifically detected authentic MARV antigen and that the sensitivity of the Ag-capture ELISA was at a level similar to that of reverse-transcription polymerase chain reaction. These results suggest that the Ag-capture ELISA using the monoclonal antibodies, 2A7 and 2H6, is applicable to the diagnosis of MHF.
机译：近期在刚果民主共和国和安哥拉爆发了由马尔堡病毒（MARV）引起的马尔堡出血热（MHF）大爆发。迫切需要开发可靠的MHF诊断系统。描述了一种抗原捕获酶联免疫吸附测定法（Ag-capture ELISA），该方法使用通过用MARV重组核蛋白免疫小鼠产生的两种单克隆抗体（2A7和2H6）中的任一种（Journal of Medical Virology，76，111-118， 2005年）。在本研究中，揭示了Ag捕获ELISA特异性地检测到真实的MARV抗原，并且Ag捕获ELISA的敏感性处于与逆转录聚合酶链反应相似的水平。这些结果表明，使用单克隆抗体2A7和2H6进行的Ag捕获ELISA可用于MHF的诊断。

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