...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Comparison of broth macrodilution, broth microdilution, and E test antifungal susceptibility tests for fluconazole.
【24h】

Comparison of broth macrodilution, broth microdilution, and E test antifungal susceptibility tests for fluconazole.

机译：肉汤大量稀释，肉汤微量稀释和氟康唑E检验抗真菌药敏试验的比较。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A comparison of the E test, the broth microdilution test, and the reference broth macrodilution susceptibility test of the National Committee for Clinical Laboratory Standards for fluconazole susceptibility testing was performed with 238 clinical isolates of Candida species and Torulopsis (Candida) glabrata. An 80% inhibition endpoint MIC was determined by the reference broth macrodilution method after 48 h of incubation. The MICs obtained by the two study methods were read after 24 and 48 h of incubation. Overall, excellent agreement within 2 doubling dilutions was obtained between the broth microdilution and the broth macrodilution methods for the combined results for all species at both 24 h (93%) and 48 h (94%). The correlation of 24-h MIC endpoints between the E test and the broth macrodilution methods was 37% for T. glabrata, 56% for Candida tropicalis, 93% for Candida albicans, and 90% for other Candida species. The percent agreement at 48 h ranged from 34% for T. glabrata to 97% for Candida species other than C. albicans and C. tropicalis. These initial results support the further evaluation of the E test as an alternative method for fluconazole susceptibility testing of Candida species.
机译：全国238例氟康唑敏感性试验的临床实验室标准委员会对E检验，肉汤微量稀释试验和参考肉汤宏观稀释敏感性试验进行了比较，对238种念珠菌和光滑拟南芥（Candida glabrata）进行了临床分离。孵育48小时后，通过参考肉汤大量稀释法确定80％的抑制终点MIC。孵育24和48小时后，读取通过两种研究方法获得的MIC。总体而言，肉汤微量稀释和肉汤大量稀释方法在2倍稀释度内获得了极好的一致性，所有物种在24小时（93％）和48小时（94％）的合并结果。 E试验和肉汤大量稀释方法之间的24小时MIC终点之间的相关性是：毛毛圆球菌为37％，热带念珠菌为56％，白色念珠菌为93％，其他念珠菌物种为90％。在48小时时的一致性百分比范围从T. glabrata的34％到白色念珠菌和热带念珠菌以外的假丝酵母物种的97％。这些初步结果支持对E试验的进一步评估，以此作为对假丝酵母菌进行氟康唑药敏试验的替代方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号