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首页> 外文期刊>Journal of Clinical Microbiology >Clinical Evaluation of the ASTY Colorimetric Microdilution Panel for Antifungal Susceptibility Testing
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Clinical Evaluation of the ASTY Colorimetric Microdilution Panel for Antifungal Susceptibility Testing

机译：ASTY比色微量稀释板抗真菌药敏试验的临床评估

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A method using a commercially prepared colorimetric microdilution panel (ASTY; Kyokuto Pharmaceutical Industrial Co., Ltd.) was compared in four different laboratories with the National Committee for Clinical Laboratory Standards (NCCLS) reference microdilution method by testing 802 clinical isolates of Candida spp. (C. albicans, C. glabrata, C. tropicalis,C. parapsilosis, C. krusei, C. lusitaniae, C. guilliermondii, C. lipolytica, C. rugosa, and C. zeylanoides) against amphotericin B, 5-fluorocytosine (5FC), fluconazole, and itraconazole. Reference MIC endpoints were established after 48 h of incubation, and ASTY endpoints were established after 24 and 48 h of incubation. ASTY endpoints were determined to be the time at which the color of the first well changed from red (indicating growth) to purple (indicating growth inhibition) or blue (indicating no growth). Excellent agreement (within 2 dilutions) between the reference and colorimetric MICs was observed. Overall agreement was 93% at 24 h and 96% at 48 h. Agreement ranged from 90% with itraconazole and 5FC to 96% with amphotericin B at 24 h and from 92% with itraconazole to 99% with amphotericin B and 5FC at 48 h. The ASTY colorimetric microdilution panel method appears to be comparable to the NCCLS reference method for testing the susceptibilities of Candida spp. to a variety of antifungal agents.
机译：通过测试802种临床分离株，在四个不同的实验室中将使用市售比色微量稀释板（ASTY; Kyokuto Pharmaceutical Industrial Co.，Ltd.）的方法与美国临床实验室标准委员会（NCCLS）参考微量稀释方法进行了比较。念珠菌 spp。 （白色念珠菌， glabrata ， C.tropicis ， C。parapsilosis ， C。 krusei ，卢西塔角梭菌，古吉尔蒙德氏梭菌，脂解衣梭菌，皱纹梭菌，和 C。zeylanoides ）针对两性霉素B，5-氟胞嘧啶（5FC），氟康唑和伊曲康唑。孵育48小时后建立参考MIC端点，孵育24和48小时后建立ASTY端点。 ASTY终点被确定为第一口井的颜色从红色（指示生长）变为紫色（指示生长抑制）或蓝色（指示无生长）的时间。观察到参比MIC与比色MIC极佳的一致性（在2个稀释度之内）。总体一致性在24小时为93％，在48小时为96％。在24小时内，伊曲康唑和5FC的一致性从90％到两性霉素B的96％，在两小时内伊曲康唑的92％到两性霉素B和5FC的99％不等。 ASTY比色微量稀释面板法似乎可以与NCCLS参考方法用于测试 Candida spp的药敏性。各种抗真菌剂。

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