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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Three Commercially Available Peptide-Based Immunoglobulin G (IgG) and IgA Assays to Microimmunofluorescence Assay for Detection of Chlamydia trachomatis Antibodies
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Comparison of Three Commercially Available Peptide-Based Immunoglobulin G (IgG) and IgA Assays to Microimmunofluorescence Assay for Detection of Chlamydia trachomatis Antibodies

机译：三种市售的基于肽的免疫球蛋白G（IgG）和IgA检测方法与用于检测沙眼衣原体抗体的微免疫荧光检测方法的比较

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Three commercially available, peptide-based enzyme-linked immunosorbent assay (ELISA) systems (Chlamydia trachomatis IgG and IgA EIA [CT-EIA; Labsystems OY, Helsinki, Finland], SeroCT IgG and IgA [SeroCT; Savyon Diagnostics Ltd., Ashdod, Israel], and Chlamydia trachomatis IgG and IgA pELISA [CT pELISA; Medac, Wedel, Germany]) were evaluated for the detection of serum immunoglobulin G (IgG) and IgA antibodies specific for Chlamydia trachomatis and compared to the “gold standard” assay, the microimmunofluorescence (MIF) assay. Serological responses were analyzed in 149 women aged 20 to 30 years. Cervical swabs obtained from these women were examined for C. trachomatis by PCR, and 43 were found to be positive. The overall seroprevalence rates detected by CT-EIA, SeroCT, CT pELISA, and the MIF assay were 42, 42, 35, and 39%, respectively, for IgG and 7, 7, 3, and 7%, respectively, for IgA. The IgG seroprevalence rates for the PCR-positive women were two to three times higher than those for the PCR-negative women, i.e., 72 versus 29%, 72 versus 29%, 47 versus 26%, and 74 versus 25% for CT-EIA, SeroCT, CT pELISA, and the MIF assay, respectively. After discrepancy analysis, the sensitivity, specificity, positive predictive value, and negative predictive value were calculated for the IgG assays; for CT-EIA they were 84.7, 98.6, 98.4, and 86.7%, respectively; for CT pELISA they were 71.4, 97.3, 96.2, and 78.3%, respectively; for SeroCT they were 84.7, 98.6, 98.4, and 86.3%, respectively; and for the MIF assay they were 79.2, 83.1, 98.3, and 83.1%, respectively. In conclusion, these peptide-based ELISA systems for the serological detection of C. trachomatis infection performed as well as the MIF assay. Since these tests are less time-consuming, less expensive, and easier to perform than the MIF assay, they might be useful in the serodiagnosis of chlamydial infection.
机译：三种市售的基于肽的酶联免疫吸附测定（ELISA）系统（沙眼衣原体IgG和IgA EIA [CT-EIA; Labsystems OY，赫尔辛基，芬兰]，SeroCT IgG和IgA [SeroCT; Savyon Diagnostics Ltd.，阿什杜德，以色列]和沙眼衣原体IgG和IgA pELISA [CT pELISA; Medac，Wedel，德国]）被评估用于检测沙眼衣原体的血清免疫球蛋白G（IgG）和IgA抗体，并与“金标准”检测，微免疫荧光（MIF）检测。对149名20至30岁妇女的血清学反应进行了分析。检查从这些妇女获得的宫颈拭子的C。 PCR检测到沙眼菌，阳性43例。通过CT-EIA，SeroCT，CT pELISA和MIF分析检测到的总体血清阳性率，IgG分别为42％，42％，35％和39％，IgA分别为7％，7％，3％和7％。 PCR阳性女性的IgG血清阳性率是PCR阴性女性的2-3倍，即CT-女性分别为72％对29％，72％对29％，47对26％，74对25％。 EIA，SeroCT，CT pELISA和MIF分析。经过差异分析后，计算出IgG检测的灵敏度，特异性，阳性预测值和阴性预测值。 CT-EIA分别为84.7、98.6、98.4和86.7％; CT pELISA分别为71.4％，97.3％，96.2和78.3％;对于SeroCT，分别为84.7％，98.6、98.4和86.3％;对于MIF分析，分别为79.2％，83.1、98.3和83.1％。总之，这些基于肽的ELISA系统可用于 C的血清学检测。进行沙眼感染以及MIF检测。由于与MIF分析相比，这些测试耗时少，价格便宜且易于执行，因此它们可能在衣原体感染的血清学诊断中很有用。

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