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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of the APTIMA CT and GC Assays with the APTIMA Combo 2 Assay, the Abbott LCx Assay, and Direct Fluorescent-Antibody and Culture Assays for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
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Comparison of the APTIMA CT and GC Assays with the APTIMA Combo 2 Assay, the Abbott LCx Assay, and Direct Fluorescent-Antibody and Culture Assays for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae

机译：将APTIMA CT和GC分析与APTIMA Combo 2分析，Abbott LCx分析以及直接荧光抗体和培养法检测沙眼衣原体和淋病奈瑟氏菌的比较

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The Gen-Probe APTIMA Combo 2 (AC2) is a Food and Drug Administration-cleared nucleic acid amplification test (NAAT) for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae from urine and urogenital swab specimens. The Centers for Disease Control and Prevention have recommended confirmation of positive NAAT results in low-prevalence populations. APTIMA CT (ACT) and APTIMA GC (AGC) are two discrete NAATs for C. trachomatis and N. gonorrhoeae detection that are suitable for confirming AC2-positive results because they target different nucleic acid sequences. Our objective was to determine if ACT and AGC could be used as confirmatory tests for AC2 and to correlate the APTIMA assays with culture, direct fluorescent-antibody (DFA), and LCx CT and GC assays. Urine and swab specimens (1,304) were initially tested with either culture, DFA, or LCx, followed by AC2. A subset (675) was then tested with ACT and AGC. There was absolute concordance between ACT-AGC and AC2. LCx did not detect 1 of 14 AC2-ACT- and 1 of 6 AC2-AGC-positive urine samples, and it yielded one C. trachomatis- and one N. gonorrhoeae-positive swab result that were not detected by AC2 and ACT-AGC. Culture failed to detect 5 of 20 AC2-ACT and 3 of 4 AC2-AGC positives, and DFA missed 4 of 4 AC2-ACT positives. Thus, ACT and AGC relative sensitivity compared to that of AC2 was 100%. All APTIMA assays detected more confirmed positive results than culture, DFA, and LCx. The performance of APTIMA assays was not altered by the use of various swab types and by long-term storage of specimens. All APTIMA assays are highly sensitive and rapid. ACT and AGC can be recommended for confirmation of positive results from other NAATs, such as AC2 and LCx.
机译：Gen-Probe APTIMA Combo 2（AC2）是食品和药物管理局批准的核酸扩增试验（NAAT），用于从尿液中检测沙眼衣原体和淋病奈瑟菌。和泌尿生殖器拭子标本。疾病控制与预防中心建议在低流行人群中确认NAAT阳性。 APTIMA CT（ACT）和APTIMA GC（AGC）是 C的两个离散NAAT。沙眼和 N。淋球菌检测适用于确认AC2阳性结果，因为它们靶向不同的核酸序列。我们的目标是确定ACT和AGC是否可以用作AC2的验证性测试，并将APTIMA测定与培养，直接荧光抗体（DFA）以及LCx CT和GC测定相关联。首先使用培养物，DFA或LCx，然后使用AC2对尿液和拭子标本（1,304）进行测试。然后用ACT和AGC测试了一个子集（675）。 ACT-AGC和AC2之间存在绝对的一致性。 LCx没有检测到14个AC2-ACT-尿液中的1个和6个AC2-AGC阳性尿液样品中的1个，并且产生了1个C。沙眼-和一个 N。 AC2和ACT-AGC未检测到淋球菌阳性拭子结果。培养物未能检测出20个AC2-ACT阳性中的5个和4个AC2-AGC阳性中的3个，而DFA错过了4个AC2-ACT阳性中的4个。因此，与AC2相比，ACT和AGC的相对灵敏度为100％。与培养液，DFA和LCx相比，所有APTIMA检测法均能检测出更多证实的阳性结果。通过使用各种拭子和长期保存标本，APTIMA分析的性能没有改变。所有APTIMA分析都高度灵敏且快速。可以推荐使用ACT和AGC来确认其他NAAT（例如AC2和LCx）的阳性结果。

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