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首页> 外文期刊>Journal of Clinical Microbiology >Immune Reactivity of Human Sera to the Glycoprotein B of Human Herpesvirus 7
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Immune Reactivity of Human Sera to the Glycoprotein B of Human Herpesvirus 7

机译：人血清对人疱疹病毒7糖蛋白B的免疫反应性

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The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been assumed to induce a specific human immune response. However, it did not appear as an immunodominant protein in conventional immunoblot assays. Recombinant gB, obtained from either Escherichia coli or baculovirus expression systems, did react specifically with HHV-7-seropositive sera, and the main corresponding epitopes were located in its N-terminal part. A 24-amino-acid peptide, corresponding to a predicted hydrophilicity peak and presenting no extensive homology with other betaherpesvirus glycoproteins, was selected in this region at positions 129 to 152 of the gB sequence. When tested by enzyme-linked immunosorbent assay (ELISA), this peptide specifically reacted with HHV-7-seropositive sera. This reactivity was significantly inhibited by the preincubation of sera with the peptide itself, lysates of gB-expressing cells, or lysates of HHV-7-infected cells. The reactivity was not significantly modified when sera were preincubated with lysates of either human cytomegalovirus (HCMV)- or HHV-6-infected cells. In cross-sectional studies including both children and adults, 49 out of 61 serum samples (80%) were found to be positive by HHV-7 ELISA, independent of their reactivity to HCMV. A longitudinal serological study of 17 children during the first 4 years of life showed that the level of ELISA-detected antibodies significantly decreased within a few weeks after birth and then increased in the following months, likely reflecting, respectively, the loss of maternal antibodies and the occurrence of seroconversion. These results demonstrate that gB peptide ELISA might be a useful tool for the serological study of HHV-7 infection.
机译：糖蛋白B（gB）在不同的人类疱疹病毒7（HHV-7）株之间高度保守。与其他疱疹病毒糖蛋白相似，已假定gB会诱导特定的人类免疫反应。但是，它在常规免疫印迹分析中并未显示为免疫优势蛋白。从大肠杆菌或杆状病毒表达系统获得的重组gB确实与HHV-7血清反应阳性血清发生特异性反应，并且主要的相应表位位于其N末端。在gB序列的129至152位区域中选择了一个24个氨基酸的肽段，该肽段对应于预测的亲水性峰，并且与其他β疱疹病毒糖蛋白没有广泛的同源性。通过酶联免疫吸附测定（ELISA）进行测试时，该肽与HHV-7血清反应阳性血清发生特异性反应。血清与肽本身，表达gB的细胞的裂解物或感染HHV-7的细胞的裂解物进行预温育，可显着抑制这种反应性。当血清与人巨细胞病毒（HCMV）或HHV-6感染细胞的裂解物一起预孵育时，反应性没有明显改变。在包括儿童和成人在内的横断面研究中，通过HHV-7 ELISA发现61份血清样本中有49份（80％）呈阳性，而与HCMV的反应性无关。在出生后的头4年对17名儿童进行的纵向血清学研究表明，ELISA检测到的抗体水平在出生后几周内显着下降，然后在接下来的几个月中上升，这可能分别反映了母体抗体和血清转化的发生。这些结果表明，gB肽ELISA可能是HHV-7感染的血清学研究的有用工具。

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