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首页> 外文期刊>Journal of Clinical Microbiology >First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR
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First Case of Type E Wound Botulism Diagnosed Using Real-Time PCR

机译:使用实时荧光定量PCR诊断E型伤口肉芽肿的第一例

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Wound botulism is a growing problem among injecting drug users. The condition is often difficult to diagnose, with laboratory confirmation in only 50% of the cases. Here we present a real-time PCR-based method for the diagnosis of wound botulism caused by Clostridium botulinum. The assay includes an internal amplification control which is amplified simultaneously with the genes encoding neurotoxin types A, B, and E. This method was used to detect the first case of wound botulism in an injecting drug user in Sweden. In addition, to the best of our knowledge, this is the first reported case of wound botulism caused by C. botulinum type E.
机译:伤口肉毒中毒是注射吸毒者中日益严重的问题。这种病通常很难诊断,只有50%的病例需要实验室确认。在这里,我们提出了一种基于实时荧光定量PCR的方法来诊断肉毒梭菌引起的伤口肉毒杆菌中毒。该测定法包括一个内部扩增对照,该扩增对照与编码A,B和E型神经毒素的基因同时扩增。该方法用于检测瑞典注射吸毒者的第一例伤口肉毒中毒。此外,据我们所知,这是首例由 C引起的肉毒杆菌中毒的报道病例。 E型肉毒杆菌。

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