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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of a Rapid Molecular Method, the BD GeneOhm Cdiff Assay, to the Most Frequently Used Laboratory Tests for Detection of Toxin-Producing Clostridium difficile in Diarrheal Feces
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Comparison of a Rapid Molecular Method, the BD GeneOhm Cdiff Assay, to the Most Frequently Used Laboratory Tests for Detection of Toxin-Producing Clostridium difficile in Diarrheal Feces

机译：BD GeneOhm Cdiff快速分子方法与最常用于检测腹泻粪便中产毒素艰难梭菌的实验室测试的比较

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Six hundred diarrheal stool specimens were collected from inpatients and outpatients at local university hospitals for the detection of toxigenic Clostridium difficile using three parallel methods, the BD GeneOhm Cdiff assay, the tissue culture cytotoxicity assay, and a commercially available enzyme-linked fluorescence immunoassay (ELFA) (Vidas C. difficile toxin A and B assay; bioMérieux). Toxigenic C. difficile culture was also performed to further clarify discordant results. During a 3-month study period, 58 (9.7%) of the 600 diarrheal samples examined were positive by the BD GeneOhm Cdiff assay, while the Vidas C. difficile toxin A and B assay and the cytotoxicity assay performed directly on stool samples gave 4.7% and 6.3% positivity rates, respectively. In the case of four samples, BD GeneOhm Cdiff assay results were not evaluable at first because of the presence of PCR inhibitors, but upon repeat testing from the frozen lysates, all of these samples proved to be negative. After resolution with toxigenic culture, the cytotoxicity assay proved to be positive in 55 samples (9.2%), while the ELFA was positive in 37 samples (6.2%). Results of culture and repeated cytotoxicity assays emphasized the importance of the culture method, because the use of ELFA or enzyme immunoassay without a culture method may lead to a substantial portion of toxigenic C. difficile strains being missed.
机译：从当地大学医院的住院患者和门诊患者中收集了600份腹泻大便标本，使用BD GeneOhm Cdiff分析，组织培养细胞毒性分析和市售三种平行检测方法来检测艰难梭菌酶联荧光免疫测定（ELFA）（维达斯艰难梭菌毒素A和B测定;bioMérieux）。产毒 C。还进行了艰难梭菌培养以进一步阐明不一致的结果。在为期3个月的研究期间，通过BD GeneOhm Cdiff分析，检测到的600份腹泻样品中有58份（9.7％）呈阳性，而Vidas C呈阳性。粪便样品中艰难梭菌毒素A和B的测定以及细胞毒性测定的阳性率分别为4.7％和6.3％。在四个样品的情况下，起初由于存在PCR抑制剂而无法评估BD GeneOhm Cdiff分析结果，但是在对冷冻裂解物进行重复测试后，所有这些样品均被证实为阴性。用毒物培养物拆分后，证明其细胞毒性测定在55个样品中为阳性（9.2％），而ELFA在37个样品中为阳性（6.2％）。培养的结果和重复的细胞毒性测定法强调了培养方法的重要性，因为不使用培养法的ELFA或酶免疫测定法的使用可能会导致很大一部分产毒素的C。难分离的菌株。

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