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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli
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Evaluation of 11 PCR Assays for Species-Level Identification of Campylobacter jejuni and Campylobacter coli

机译：空肠弯曲菌和大肠杆菌弯曲菌种鉴定的11种PCR检测方法的评价

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We examined the sensitivity and specificity of 11 PCR assays described for the species identification of Campylobacter jejuni and Campylobacter coli by using 111 type, reference, and field strains of C. jejuni, C. coli, and Campylobacter lari. For six assays, an additional 21 type strains representing related Campylobacter, Arcobacter, and Helicobacter species were also included. PCR tests were initially established in the laboratory by optimizing conditions with respect to five type and reference strains of C. jejuni, C. coli, and C. lari. One PCR test for C. coli failed to give appropriate results during this initial setup phase and was not evaluated further. The remaining 10 assays were used to examine heated lysate and purified DNA templates as appropriate of well-characterized type, reference, and field strains of C. jejuni (n = 62), C. coli (n = 34), and C. lari (n = 15). The tests varied considerably in their sensitivity and specificity for their respective target species. No assay was found to be 100% sensitive and/or specific for all C. jejuni strains tested, but four assays for C. coli gave appropriate responses for all strains examined. Between one and six strains of C. jejuni gave amplicons in four of seven C. jejuni PCR tests only where purified DNA was used as the template; corresponding results were seen with one strain of C. coli in each of three assays for the latter species. Our findings indicate that a polyphasic strategy for PCR-based identification should be used to identify C. jejuni and C. coli strains. The data may assist laboratories in selecting assays suited for their needs and in designing evaluations of future PCR tests aimed to identify these species.
机译：我们使用111种类型，参考和野外菌株对空肠弯曲杆菌和<弯曲杆菌弯曲杆菌进行11种PCR检测的敏感性和特异性。空肠， C。大肠杆菌和弧形弯曲杆菌。对于六个测定，还包括代表相关的<弯曲>弯曲杆菌，<弯曲> Arcobacter 和<弯曲>螺旋杆菌物种的另外21种菌株。 PCR试验最初是在实验室中通过针对五种C型和参考菌株的条件进行优化来建立的。空肠， C。大肠杆菌和 C。拉里。一种针对 C的PCR测试。在最初的设置阶段，大肠杆菌没有给出适当的结果，因此未作进一步评估。其余的10种测定法被用来检查加热的裂解物和纯化的DNA模板（如特征明确的C，em和C型野外菌株）。 jejuni （ n = 62）， C。大肠杆菌（ n = 34）和 C。 lari （ n = 15）。这些测试对各自目标物种的敏感性和特异性差异很大。没有发现对所有C均100％敏感和/或特异的检测方法。测试了空肠菌株，但有四种测定 C的方法。大肠埃希菌对所检查的所有菌株均给出了适当的反应一到六株 C。空肠在七个 C中的四个中扩增出扩增子。空肠PCR仅在以纯化的DNA为模板的情况下进行测试;用一株 C观察到相应的结果。大肠埃希菌的三种测定中的每一种。我们的发现表明基于PCR的鉴定的多相策略应用于鉴定 C。空肠和 C。大肠杆菌菌株。数据可以帮助实验室选择适合其需要的分析方法，并设计用于鉴定这些物种的未来PCR测试的评估。

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