Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application

Yixiang Jiang; Lin Fang; Xiaolu Shi; Hailong Zhang; Yinghui Li; Yiman Lin; Yaqun Qiu; Qingliang Chen; Hui Li; Li Zhou; Qinghua Hu

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Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application

机译：PCR检测同时检测与肠胃炎相关的五种肠病毒：单一实时多重反应及其临床应用

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We developed a highly sensitive reverse transcription and multiplex real-time PCR (rtPCR) assay that can identify five viruses, including six genogroups, in a single reaction: norovirus genogroups I and II; sapovirus genogroups I, II, IV, and V; human rotavirus A; adenovirus serotypes 40 and 41; and human astrovirus. In comparison to monoplex rtPCR assays, the sensitivities and specificities of the multiplex rtPCR ranged from 75% to 100% and from 99% to 100%, respectively, evaluated on 812 clinical stool specimens.

机译：我们开发了一种高度灵敏的逆转录和实时荧光定量PCR（rtPCR）分析方法，可在一次反应中鉴定出五种病毒，包括六个基因组：诺如病毒基因组I和II;沙波病毒基因组I，II，IV和V;人类轮状病毒A;腺病毒血清型40和41;和人类星状病毒。与单重rtPCR分析相比，对812个临床粪便标本进行评估，多重rtPCR的敏感性和特异性分别为75％至100％和99％至100％。

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《Journal of Clinical Microbiology》 |2014年第4期|共3页
作者
Yixiang Jiang; Lin Fang; Xiaolu Shi; Hailong Zhang; Yinghui Li; Yiman Lin; Yaqun Qiu; Qingliang Chen; Hui Li; Li Zhou; Qinghua Hu;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. A modified molecular beacons-based multiplex real-time PCR assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application [J] . HuQ., LyuD., ShiX., Foodborne pathogens and disease . 2014,第3期

机译：改进的基于分子信标的多重实时PCR分析法，可同时检测单个反应中的八个食源性病原体及其应用
2. A modified molecular beacons-based multiplex real-time PCR assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application [J] . HuQ., LyuD., ShiX., Foodborne pathogens and disease . 2014,第3期

机译：基于修饰的分子信标的多重实时PCR测定，用于在单一反应中同时检测八种食源性病原体及其应用
3. Development of a multiplex real‐time PCR assay for detection of human enteric viruses other than norovirus using samples collected from gastroenteritis patients in Fukui Prefecture, Japan [J] . Kowada Kazuaki, Takeuchi Kenji, Hirano Eiko, Journal of Medical Virology . 2018,第1期

机译：使用日本福井县胃肠炎患者收集的样品，检测多重实时PCR测定的多重实时PCR测定检测诺病毒以外的样品
4. Real-time multiplex PCR-assay for simultaneous detection and differentiation of beverage spoilage bacteria and yeasts [C] . Jutta Schoenling, Gabriele Muecher, Ulrike Erxlebe European Brewery Convention Congress . 2007

机译：实时多重PCR检测可同时检测和区分饮料中的腐败菌和酵母菌
5. Rapid detection of “Norwalk-like viruses” by reverse transcription-polymerase chain reaction (RT-PCR) and southern blot hybridisation (SBH) in outbreaks of acute gastroenteritis in eastern Ontario, Canada [D] . Wires, Shannon Meredith 2001

机译：通过逆转录聚合酶链反应（RT-PCR）和Southern blot杂交（SBH）快速检测加拿大安大略省东部急性肠胃炎暴发中的“ Norwalk样病毒”
6. Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application [O] . Yixiang Jiang, Lin Fang, Xiaolu Shi, 2014

机译：PCR检测同时检测与肠胃炎相关的五种肠病毒：单一实时多重反应及其临床应用
7. Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application [O] . Y. Jiang, L. Fang, X. Shi, 2014

机译：通过使用PCR测定同时检测与胃肠炎相关的五种肠道病毒：单一的实时多重反应及其临床应用

Simultaneous Detection of Five Enteric Viruses Associated with Gastroenteritis by Use of a PCR Assay: a Single Real-Time Multiplex Reaction and Its Clinical Application

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