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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Statens Serum Institut Enteric Medium for Detection of Enteric Pathogens
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Evaluation of Statens Serum Institut Enteric Medium for Detection of Enteric Pathogens

机译：评估州立大学血清研究所肠溶介质检测肠病原菌的价值

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The efficacy of the Statens Serum Institut (SSI) enteric medium for isolation and direct identification of enteric pathogens was evaluated. Six different biochemical reactions can be read by using the SSI enteric medium, allowing direct identification of a range of enteric pathogens. All 248 gram-negative bacterial species that were tested grew on the SSI enteric medium. Only 10 of 248 bacteria (4%) showed discrepant results in the biochemical reactions, and none of these were enteric pathogens. Forty-three of 47 enteric pathogens (92%) produced identical rates of semiquantitative growth on the SSI enteric medium and 5% blood agar, whereas three Vibrio spp. and oneAeromonas spp. showed reduced growth. Gram-positive bacteria did not grow on the SSI enteric medium. Most enteric pathogens had a detection limit of 50 bacteria per ml of feces, but higher numbers of Vibrio spp. and some Shigella spp. were required for detection. The growth rates of 125 enteric pathogens and 12 Yersinia spp. on the SSI enteric medium, xylose lysine deoxycholate (XLD), Hektoen enteric (HE),Salmonella-Shigella (SS), and cefsulodin-irgasan-novobiocin (CIN) agar were compared. Detection rates after application of 200 CFU were 99% for SSI enteric medium, 92% for XLD, 88% for HE, and 82% for SS agar. The 12 Yersinia spp. grew excellently on both the SSI enteric medium and CIN agar. We conclude that the performance of the SSI enteric medium compares favorably to those of other media tested. Its ability to detect Yersinia spp. may limit the number of media needed in the typical laboratory. The direct identification of enteric pathogens on the medium may also provide a more rapid diagnosis.
机译：评估了史坦顿血清研究所（SSI）肠溶介质对肠道病原体分离和直接鉴定的功效。通过使用SSI肠溶介质，可以读取六种不同的生化反应，从而可以直接识别一系列肠溶病原体。所有被测试的248克阴性细菌都在SSI肠溶培养基上生长。 248个细菌中只有10个（4％）在生化反应中显示出不同的结果，而这些都不是肠内病原体。 47种肠道病原体中有43种（92％）在SSI肠溶培养基和5％血琼脂上产生了相同的半定量生长速率，而三种 Vibrio spp。和一个 Aeromonas spp。显示增长减少。革兰氏阳性细菌未在SSI肠溶培养基上生长。大多数肠道病原体的检出限为每毫升粪便中有50个细菌，但弧菌 spp的数量更高。还有一些志贺氏菌 spp。需要进行检测。 125种肠道病原体和12种耶尔森氏菌的生长速率。在SSI肠溶培养基上，比较了木糖赖氨酸脱氧胆酸盐（XLD），Hektoen肠溶（HE），沙门氏菌-志贺氏菌（em）和头孢磺啶-依加根-新生物素（CIN）琼脂。施用200 CFU后，SSI肠溶培养基的检出率为99％，XLD为92％，HE为88％，SS琼脂为82％。 12个耶尔森氏菌属。在SSI肠溶培养基和CIN琼脂上均生长良好。我们得出的结论是，SSI肠溶介质的性能优于其他测试介质。其检测耶尔森氏菌 spp的能力。可能会限制典型实验室中所需的介质数量。在培养基上直接鉴定肠病原体也可以提供更快的诊断。

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