Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection

David W. C. Beasley; Michael R. Holbrook; Amelia P. A. Travassos da Rosa; Lark Coffey; Anne-Sophie Carrara; Kathrine Phillippi-Falkenstein; Rudolf P. Bohm; Marion S. Ratterree; Kristy M. Lillibridge; George V. Ludwig; Jose Estrada-Franco; Scott C. Weaver; Robert B. Tesh; Robert E. Shope; Alan D. T. Barrett

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Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection

机译：重组信封蛋白亚基抗原在西尼罗河病毒感染的特异性血清学诊断中的应用

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Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.

机译：与其他密切相关的黄病毒，例如圣路易斯脑炎病毒的广泛抗原交叉反应，使血清学诊断西尼罗河病毒（WNV）变得复杂。在这里，我们描述了一种重组的，细菌表达的抗原，其等效于WNV包膜蛋白的结构域III，在使用标准化对照抗血清和现场收集的间接酶联免疫吸附测定中，已明确区分了针对WNV的抗体反应与针对其他相关黄病毒的抗体反应样品。

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《Journal of Clinical Microbiology》 |2004年第6期|共7页
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David W. C. Beasley; Michael R. Holbrook; Amelia P. A. Travassos da Rosa; Lark Coffey; Anne-Sophie Carrara; Kathrine Phillippi-Falkenstein; Rudolf P. Bohm; Marion S. Ratterree; Kristy M. Lillibridge; George V. Ludwig; Jose Estrada-Franco; Scott C. Weaver; Robert B. Tesh; Robert E. Shope; Alan D. T. Barrett;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. Immunization of Flavivirus West Nile Recombinant Envelope Domain III Protein Induced Specific Immune Response and Protection against West Nile Virus Infection. [J] . Chu JH, Chiang CC, Ng ML The Journal of Immunology: Official Journal of the American Association of Immunologists . 2007,第5期

机译：黄病毒西尼罗河重组信封域III蛋白的免疫诱导特异性免疫反应和针对西尼罗河病毒感染的保护。
2. Immunization of Flavivirus West Nile Recombinant Envelope Domain III Protein Induced Specific Immune Response and Protection against West Nile Virus Infection [J] . Jang-Hann J. Chu, Cern-Cher S. Chiang, Mah-Lee Ng The journal of immunology . 2007,第5期

机译：黄病毒西尼罗河重组信封域III蛋白的免疫诱导特异性免疫反应和针对西尼罗河病毒感染的保护。
3. Induction of antigen-specific immune responses in mice by recombinant baculovirus expressing premembrane and envelope proteins of West Nile virus [J] . Bibo Zhu, Jing Ye, Ping Lu, Virology Journal . 2012,第1期

机译：表达西尼罗河病毒前膜和包膜蛋白的重组杆状病毒诱导小鼠抗原特异性免疫反应
4. Evaluation of the Efficacy Provided by a Recombinant Canarypox-Vectored Equine West Nile Virus Vaccine Against an Experimental West Nile Virus Intrathecal Challenge in Horses [C] . Leonardo Siger, Richard A. Bowen, Kemal Karaca, Annual Convention of the American Association of Equine Practitioners . 2007

机译：评估重组Canarypox的疗效，将马西尼亚尼罗河病毒疫苗对马匹的实验西尼罗河病毒鞘内挑战进行评估
5. The contribution of West Nile virus envelope protein N-linked glycosylation on pathogenesis. [D] . Hanna, Sheri L. 2007

机译：西尼罗河病毒包膜蛋白N-联糖基化对发病的贡献。
6. Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection [O] . David W. C. Beasley, Michael R. Holbrook, Amelia P. A. Travassos da Rosa, 2004

机译：重组信封蛋白亚基抗原在西尼罗河病毒感染的特异性血清学诊断中的应用
7. Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection [O] . Beasley, David W. C., Holbrook, Michael R., Travassos da Rosa, Amelia P. A., 2004

机译：重组信封蛋白亚基抗原在西尼罗河病毒感染的特异性血清学诊断中的应用
8. Simultaneous Confirmation and Differentiation of Human T-lymphotropic Virus TypesI and II Infections by Modified Western Blot Containing Recombinant Envelope Glycoproteins [R] . Brodine, S. K., Kaime, E. M., Roberts, C., 1993

机译：通过含有重组包膜糖蛋白的改良蛋白质印迹同时确认和分化人类T淋巴细胞病毒I型和II型感染

Use of a Recombinant Envelope Protein Subunit Antigen for Specific Serological Diagnosis of West Nile Virus Infection

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